Techdata Service Company LLC

Job Description Job Description Contract Role 100 % Onsite Up to $60/hr. W2 Position Summary The Lead Discovery and Structural Biology Department within client is seeking a highly qualified Protein Expression & Purification Scientist (Contractor) with demonstrated expertise in molecular biology , heterologous protein expression , and protein purification . The successful candidate will contribute to research programs within an industry-leading drug discovery environment leveraging access to state-of-the-art protein expression and purification platforms . Responsibilities include executing expression workflows in baculovirus expression vector systems (BEVS)/insect cells , as well as in E. coli, including high-throughput (HTP) small-scale expression screening , followed by downstream purification and analytical characterization. Experience with biophysical methods is considered an asset. Essential Duties and Responsibilities Perform molecular biology workflows, including: Cloning, vector design, construct optimization, and sequence verification PCR, primer design, restriction based, and recombination based cloning Execute protein expression using: BEVS/insect cell systems , including virus generation, amplification, and expression optimization E. coli expression , including IPTG induced and auto induction strategies High throughput (HTP) small scale expression testing for rapid screening of constructs and conditions Conduct purification of recombinant proteins using standard chromatographic techniques (affinity, ion exchange, size exclusion chromatography). Optimize expression constructs, culture conditions, and purification workflows to achieve the required yield, purity, and quality. Perform analytical characterization, including SDS PAGE, Western blotting, SEC, and other relevant assays. Maintain accurate and complete laboratory records in accordance with internal documentation standards (experience with Benchling is a plus). Communicate experimental results, observations, and recommendations to team members in a clear and timely manner. Adhere to all laboratory safety policies and organizational compliance requirements. Education and Experience Bachelor's degree in Biochemistry, Molecular Biology, Structural Biology, or related field with 3+ years of relevant industry experience, or Master's degree with 1+ year of relevant industry experience Strong molecular biology and cloning expertise is required. Proven track record of protein expression in BEVS/insect systems and E. coli, including HTP small scale expression screening. Demonstrated proficiency in chromatographic purification and method development. Experience with biophysical characterization techniques (e.g., LC MS, SPR, MST, SEC MALS, nanoDSF, mass photometry) is preferred but not required. Company Description Company Description

Job Description Job Description Contract Role 100 % Onsite Up to $60/hr. W2 Position Summary The Lead Discovery and Structural Biology Department within client is seeking a highly qualified Protein Expression & Purification Scientist (Contractor) with demonstrated expertise in molecular biology , heterologous protein expression , and protein purification . The successful candidate will contribute to research programs within an industry-leading drug discovery environment leveraging access to state-of-the-art protein expression and purification platforms . Responsibilities include executing expression workflows in baculovirus expression vector systems (BEVS)/insect cells , as well as in E. coli, including high-throughput (HTP) small-scale expression screening , followed by downstream purification and analytical characterization. Experience with biophysical methods is considered an asset. Essential Duties and Responsibilities Perform molecular biology workflows, including: Cloning, vector design, construct optimization, and sequence verification PCR, primer design, restriction based, and recombination based cloning Execute protein expression using: BEVS/insect cell systems , including virus generation, amplification, and expression optimization E. coli expression , including IPTG induced and auto induction strategies High throughput (HTP) small scale expression testing for rapid screening of constructs and conditions Conduct purification of recombinant proteins using standard chromatographic techniques (affinity, ion exchange, size exclusion chromatography). Optimize expression constructs, culture conditions, and purification workflows to achieve the required yield, purity, and quality. Perform analytical characterization, including SDS PAGE, Western blotting, SEC, and other relevant assays. Maintain accurate and complete laboratory records in accordance with internal documentation standards (experience with Benchling is a plus). Communicate experimental results, observations, and recommendations to team members in a clear and timely manner. Adhere to all laboratory safety policies and organizational compliance requirements. Education and Experience Bachelor's degree in Biochemistry, Molecular Biology, Structural Biology, or related field with 3+ years of relevant industry experience, or Master's degree with 1+ year of relevant industry experience Strong molecular biology and cloning expertise is required. Proven track record of protein expression in BEVS/insect systems and E. coli, including HTP small scale expression screening. Demonstrated proficiency in chromatographic purification and method development. Experience with biophysical characterization techniques (e.g., LC MS, SPR, MST, SEC MALS, nanoDSF, mass photometry) is preferred but not required. Company Description Company Description