4D Molecular Therapeutics

Summary 4DMT is in need of a talented and motivated Senior Research Associate to join our Bioinformatics team in supporting our viral drug therapy research efforts as we contribute to the future of gene therapeutics. The position will combine modern molecular and cell biology methods with scalable production processes to inform and improve discovery and experimental design in conjunction with 4DMT's proprietary discovery platform. This position will involve new protocol development and execution in the areas of AAV plasmid construction, high-throughput AAV production, purification and characterization, and cell-based optimization of payload biology. This highly-collaborative position will require close interaction on multiple projects within the Bioinformatics group to explore the wetlab aspects of computationally-derived hypotheses, and across the Discovery & Engineering department, including capsid discovery, payload engineering, and viral manufacturing. This position will also collaborate on cross-functional projects with additional groups within R&D and across the company, including HCDM, CMC, and Translational Medicine. As part of this cross-functional work, employee will be expected to attend and actively engage in working group and project team meetings. Responsibilities Research - 100% of Time Demonstrating innovative design, development, and execution of research projects through expertise and scientific teamwork Contributing to the design, implementation, and interpretation of the results of experimental studies Leveraging a strong understanding of gene therapy principles to design, assess and interpret experimental data, then using the results to inform the selection of the optimal product strategy Maintaining current awareness of scientific literature Actively applying new concepts and technologies Leading and supporting research initiatives such as new technology development and platform improvement projects Writing study reports and manuscripts and presenting results at scientific meetings Molecular biology in support of: AAV Library construction Monitoring of selections Novel screening strategies Next-generation sequencing (NGS) sample prep and MiSeq operation Nextera resequencing of plasmids and AAV genomes Refine and execute proprietary NGS-based assays for AAV sequence integrity and purity Maintaining compliance with company Environmental Health and Safety policies, procedures, and practices Contributing to building a culture that embraces continuous learning, improvement, and innovation and encourages team members to expand their technical skill base and deepen their gene therapy expertise. Qualifications BS/MS with a molecular and/or cell biology focus Experience: 5+ years industry experience Strong molecular biology experience, both hands-on as well as the ability to design and analyze sequence data using common software such as Geneious Extensive cell culture experience, with work in a plate-based format a plus Familiarity with NGS sample prep a plus Skills: (non-technical and technical skills) Successful demonstrated ability to conduct research with strong attention to detail and rigorous analysis and interpretation of data Ability to handle large-scale and parallel experiments in an organized and efficient manner Ability to multi-task and support more than one project simultaneously Highly organized and motivated; strong analytical, organizational, and problem-solving skills Strong work ethic, flexibility, and cooperative can-do attitude Proven interest and ability to build a culture that embraces continuous learning, improvement, and innovation Ability to work cooperatively in a team-based environment; supportive of multiple viewpoints and approaches Strong communication skills (both written and oral) with demonstrated ability to present ideas, information and data effectively via one on one discussions, team meetings, and external partnership interactions Physical Requirements of the Role: During COVID-19 will this person need to be in a lab? Yes How many days will be required? Full time Adhere to 4DMT Covid protocols and policy 4DMT provides equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, gender, sexual orientation, national origin, age, disability, genetic information, marital status, status as a covered veteran and any other category protected under applicable federal, state, provincial and local laws. Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities

4D Molecular Therapeutics (4DMT) is a gene therapy company with a transformative discovery platform- Therapeutic Vector Evolution -that enables our "disease first" approach to product discovery and development, thereby allowing us to customize our AAV vectors to target specific tissue types associated with the underlying disease. 4DMT is focused on attracting and retaining the best people in the industry who identify with our mission and innovative results-driven approach. We offer an inspiring and collaborative environment that encourages individual contributions, recognizes performance in multiple ways, offers uniquely generous benefits, and provides challenging opportunities for career development. Join us in creating the ultimate gene therapy products to cure genetic diseases in children and adults. 4D Molecular Therapeutics, located in Emeryville, CA. GENERAL SUMMARY: 4D is in need of a talented and motivated scientist and Biomarker specialist to join a growing group of scientists developing assays for pre-clinical pharmacology and toxicology studies and clinical trials. The role will involve considerable cross-functional interactions with our vector discovery and payload engineering groups, translational, clinical medicine and our manufacturing group. RESPONSIBILITIES: Develop and design cutting edge, innovative bioassays for preclinical and clinical use in multiple therapeutics areas, e.g. neuromuscular and lung, through expertise, and scientific teamwork General areas of biomarker development will be in genomic and gene expression, immunologic, protein and histologic and cell-based assays Designing, executing, and analyzing data using next-gen analytical techniques Contributing to the design, implementation, and interpretation of the results of in vivo and in vitro experimental studies Assessing the utility and selection of contract research organizations (CROs), technology transfer, overseeing assay method development, qualification and validation Performing hands-on research in the company's lab to develop in house bioassays Potential for contribution to analytical development bioassays Leveraging a strong understanding of bioassays, assess and interpret experimental data, then using the resulting information to inform the further product development Effectively mentoring junior level staff and acting as a resource for scientific expertise Utilizing appropriate scientific approaches to understand characteristics of gene expression and protein function to drive decisions and assess potential application of company's lead adeno-associated virus vectors and products Maintaining current awareness of scientific literature Actively applying new concepts and technologies Leading and supporting research initiatives such as new technology development and platform improvement projects Writing study reports and manuscripts and presenting results at scientific meetings Maintaining compliance with company Environmental Health and Safety policies, procedures, and practices Contributing to building a culture that embraces continuous learning, improvement, and innovation and encourages team members to expand their technical skill base and deepen their gene therapy expertise. QUALIFICATIONS: Education: Bachelors in Bioengineering, Molecular Biology, Cell Biology, Biochemistry or related field Experience: With a PhD - 4+ years of industry experience (Masters with 6+, Bachelors with 8+ Years), biomarker/bioassay development strongly preferred Experience in one of the following disciplines: Bioengineering, Molecular Biology, Cell Biology, Biochemistry, or a related field Skills: Successful demonstrated ability to work independently in designing and developing novel gene regulatory elements and engineered protein variants. Successful demonstrated ability to work independently in designing, developing, and executing new assay methods Successful demonstrated ability to conduct research with strong attention to detail and rigorously analyze and interpret data Ability to multi-task and support more than one project simultaneously Highly organized and motivated; strong analytical, organizational, and problem-solving skills Strong work ethic, flexibility, and cooperative can-do attitude Ability to work cooperatively in a team-based environment; supportive of multiple viewpoints and approaches Strong communication skills (both written and oral) with demonstrated ability to present ideas, information and data effectively via one on one discussions, team meetings, and external partnership interactions Understanding and knowledge of key scientific software programs (including Microsoft Office, JMP or Prism, Adobe Photoshop, and bioinformatics and protein structure applications such as Swiss-Pdb Viewer). 4DMT provides equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, gender, sexual orientation, national origin, age, disability, genetic information, marital status, status as a covered veteran and any other category protected under applicable federal, state, provincial and local laws. Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities

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4D Molecular Therapeutics (4DMT) is a gene therapy company with a transformative discovery platform-Therapeutic Vector Evolution-that enables our "disease first" approach to product discovery and development, thereby allowing us to customize our AAV vectors to target specific tissue types associated with the underlying disease. 4DMT is focused on attracting and retaining the best people in the industry who identify with our mission and innovative results-driven approach. We offer an inspiring and collaborative environment that encourages individual contributions, recognizes performance in multiple ways, offers uniquely generous benefits, and provides challenging opportunities for career development. Join us in creating the ultimate gene therapy products to cure genetic diseases in children and adults. 4D Molecular Therapeutics, located in Emeryville, CA. GENERAL SUMMARY: 4DMT is in need of a motivated, team-oriented, and experienced Manufacturing Associate. This individual will join a small but skilled manufacturing team to deliver novel AAV vectors for gene therapy clinical trials. The Manufacturing Associate will interface with Process Development to transfer new candidates into GMP production, and provide support for experiments as needed. RESPONSIBILITIES: Execute downstream and fill finish unit operations for the manufacture of AAV vector products in a cGMP environment, using proper aseptic technique, good documentation practices, and sound scientific methods. Operations include: Column chromatography purification (AKTA Pure, Avant, Pilot systems) Depth filtration and tangential flow filtration Formulation and filling of drug product Manual visual inspection Solve operational tasks in the operation of purification processes, including column packing, UNICORN method writing, filtration assembly design, and aseptic filling via manual and automated processes. Cross-trained to different operational areas, e.g., buffer/ media preparation and upstream cell culture of adherent and suspension cells. Support the Process Development team in transfer of new projects and scale-up of processes into GMP manufacturing. Identify and elevate processing issues (large and small) and implement solutions. Provide feedback and/or suggested changes to operational procedures. Complete batch production records under cGMP & tech transfer systems to meet both operational and compliance requirements. Contribute to operational protocol(s) and internal documents including SOPs, master batch records, deviations. Troubleshoot downstream/ fill finish processes and equipment problems; work with Facilities to maintain calibration, maintenance and operation requirements of manufacturing equipment. Perform environmental monitoring activities during aseptic processing. Work closely with supervisors for setting the manufacturing schedule to meet timelines and avoid operational delays. Work closely with Materials Management to keep the downstream and fill finish areas stocked with all raw materials/ process inputs. Participate in new facility expansion and equipment validation activities as needed. Have a strong understanding of GMP procedure for clinical production of complex biologics. Follow all QA procedures, including deviation reporting. Support deviation investigations. Shift and/ or weekend work may be required QUALIFICATIONS: Education: BS in bioprocessing, chemical engineering, biochemistry or biology preferred Biotechnology program certifications considered, as well as experience providing technician support in an academic setting. Experience: At least 2 years of of cGMP manufacturing experience in biopharmaceutical industry Ability to work in a small company environment Skills: Familiarity with cGMP requirements is essential. Knowledge of affinity, ion exchange, and/ or multi-modal purification processes. Aseptic gowning/ cleanroom experience is highly desired. Familiarity UNICORN software and/ or designing single-use assemblies is preferred ·Excellent time management skills and the ability to multi-task and adapt to changing priorities. Ability to thrive and succeed in a team-oriented entrepreneurial environment, and provide leadership in a growing organization. Proficient with Excel, Word, and PowerPoint. Excellent written and oral communication skills. Physical Requirements Capable of strenuous, repetitive, physical work, including bending, reaching, climbing stairs and ladders, kneeling and making numerous equipment connections using hands. Independently have the ability to regularly lift, dispense, push and/or pull items with an average weight of 40 pounds. pass visual acuity test and color blindness test Able to work on site during COVID pandemic and adhere to 4DMT's safety protocols 4DMT provides equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, gender, sexual orientation, national origin, age, disability, genetic information, marital status, status as a covered veteran and any other category protected under applicable federal, state, provincial and local laws. Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities

4D Molecular Therapeutics (4DMT) is a gene therapy company with a transformative discovery platform- Therapeutic Vector Evolution -that enables our "disease first" approach to product discovery and development, thereby allowing us to customize our AAV vectors to target specific tissue types associated with the underlying disease. 4DMT is focused on attracting and retaining the best people in the industry who identify with our mission and innovative results-driven approach. We offer an inspiring and collaborative environment that encourages individual contributions, recognizes performance in multiple ways, offers uniquely generous benefits, and provides challenging opportunities for career development. Join us in creating the ultimate gene therapy products to cure genetic diseases in children and adults. 4D Molecular Therapeutics, located in Emeryville, CA. GENERAL SUMMARY: The 4DMT process development team is looking for a talented scientist with experience in the design and implementation of gene and genome editing towards improving cell-based AAV production. The role allows for extensive creativity, innovation and opportunity to develop novel processes, allowing for extensive coordination within and across teams. You will work closely with your peers in downstream process development, the analytical team and colleagues in research. The group works closely with our counterparts in both manufacturing and research; exchanging scientific ideas, platform improvements and AAV expertise will be a frequent and encouraged activity. RESPONSIBILITIES: Lab & Experimental Activities: 65% of time Design studies that implement gene circuit design, homology-directed repair, single nucleotide polymorphisms, viral transgenesis and clonal selection/expansion to direct and control cell function Develop studies that mature process knowledge towards AAV structure/function and improve AAV production yields/product quality and other quality attributes Implement strategies to produce engineered cells that modulate AAV production and function Execute gene and genome edits using viral and non-viral methods, including transient transfection and electroporation Optimize transfection conditions: multiplexing engineered cell lines with improvements in media formulation, transfection adjuvants, cell and other critical process parameters Develop seed train, transfection and harvest processes with considerations towards reducing cost of goods and manufacturing Draft study designs, standard operating procedures and maintain an electronic lab notebook Cross-functional & communication : 35% of time Collaborate with process & analytical teams to screen and evaluate drug candidates across a range of indications Review and present data cross-functionally at department meetings and larger forums Maintain strong working relationships with external collaborators and/or CDMOs Contribute to a culture that embraces continuous learning, improvement and innovation Draft technical reports, contribute to IND sections and other regulatory documents Maintains current awareness and contributes to current scientific literature; actively applies new concepts and technologies QUALIFICATIONS: Education: PhD in molecular biology, cell biology virology, genetics or related field, or Master in molecular biology, cell biology, virology, genetics or related field Experience: PhD with 0-3 years (or Master's with 5+ years) relevant experience Skills: Knowledgeable and hands-on experience with nucleic sequence design, cloning and cell-incorporation Experience modulating immortalized cell lines using methods that align with cGMP manufacturing Strong problem-solving, analytical skills and creativity Experimental study design, leveraging past studies and extracting from the scientific community Strong written and oral communication skills with experience presenting scientific information Excellent time management organizational skills, with ability to handle multiple tasks simultaneously in a high-growth, fast-paced environment Work Location: This position requires the ability to work on location in the 4DMT lab at least 3-4 times a week. There may be some remote work during Shelter in Place. Once Shelter in Place has been lifted this will be full-time position at the 4DMT Emeryville location 4DMT provides equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, gender, sexual orientation, national origin, age, disability, genetic information, marital status, status as a covered veteran and any other category protected under applicable federal, state, provincial and local laws. Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities