Mammoth BioSciences OPPORTUNITY Mammoth Biosciences is seeking a highly motivated Scientist II or Senior Scientist I to join our CMC team in a hands-on, cross-functional role that blends scientific ingenuity with operational excellence. This position is critical for developing, optimizing, scaling, and characterizing LNP-based delivery production processes for Mammoth's cutting-edge gene editing platforms. The ideal candidate will thrive at the interface of process development, analytics, and manufacturingsomeone who can transition smoothly between the bench and discussions with external partners. This is a great opportunity to play a key role in advancing transformative gene editing therapies into the clinic and beyond. KEY RESPONSIBILITIES Design and optimize LNP manufacturing processes by applying Quality by Design (QbD) principles and execute Design of Experiments (DoEs) to identify and control critical process parameters (CPPs) Optimize analytical methods such as DLS, Ribogreen, HPLC-CAD, IP-RP-HPLC, and endotoxin to identify and access critical quality attributes (CQAs) Interpret analytical data to assess process robustness and guide iterative development efforts Collaborate with internal teams to support process improvements and drive innovation Clearly present scientific findings and project updates in internal meetings and to external collaborators Drive technology transfer activities with external CDMO partners by preparing comprehensive technical documentation such as development reports, process summaries, and tech transfer packages to ensure successful external manufacturing Stay current with emerging trends in LNP technologies and contribute to strategic process improvements Track inventory, plan experiments, and contribute to an organized, quality-focused lab environment REQUIRED QUALIFICATIONS Ph.D. in Chemical Engineering, Bioengineering, Pharmaceutical Sciences, Chemistry, or a related field with minimum of 3 years relevant industry experience; or M.S. with 6 years; or B.S. with 8 years Demonstrated expertise in LNP formulation and process development, including hands-on experience with microfluidic and high-shear mixing technologies, tangential flow filtration and dead-end filtration Proficiency in analytical characterization techniques for LNPs, including DLS, fluorescence-based assays and HPLC/UPLC methods (CAD, UV, IP-RP, SEC, IEX), including method development, troubleshooting, and data interpretation Experience with in-process and drug product stability studies Proven success in applying DoEs and statistical analysis tools for process optimization Experience supporting technology transfer of processes to external manufacturing environments, with a strong understanding of tech transfer best practices Demonstrated strong organizational skills A self-starter with demonstrated strong troubleshooting skills, capable of driving projects independently while contributing effectively in a collaborative, multidisciplinary environment PREFERRED QUALIFICATIONS Knowledge of regulatory expectations for CMC development Familiarity with high-throughput screening methods Exposure to cell-based assays for LNP functional characterization Familiarity with data analysis tools and statistical methods such as JMP Experience with AAV vector process development or a strong interest and adaptability to learn viral delivery systems BENEFITS Company-paid health/vision/dental benefits Unlimited vacation and generous sick time Company-sponsored meals and snacks Wellness, caregiver and ergonomics benefits 401(k) with company matching Base Salary Range: $138,000 - $178,000 per year Actual base salary will be determined by relevant professional experience, applicable skills, and internal equity. COMPANY Mammoth Biosciences is a biotechnology company focused on leveraging its proprietary ultracompact CRISPR systems to develop long-term curative therapies, as well as other applications such as decentralized precision diagnostics. Founded by CRISPR pioneer and Nobel laureate Jennifer Doudna and Trevor Martin, Janice Chen, and Lucas Harrington, the companys ultracompact proteins are designed to enable in vivo gene editing in difficult to reach tissues utilizing both nuclease applications and new editing modalities beyond double stranded breaks, including base editing, gene writing, and epigenetic editing. The company is building out its pipeline of potential in vivo gene editing therapeutics and capabilities and has partnerships with leading pharmaceutical and biotechnology companies to broaden the reach of its innovative and proprietary technology. Mammoths deep science and industry experience, along with a robust and differentiated intellectual property portfolio, have enabled the company to further its mission to transform the lives of patients and deliver on the full promise of CRISPR technologies. It is our policy and intent to provide equal opportunity to all persons without regard to race, color, religion, political affiliation, sex/gender (including gender expression/identity, pregnancy, childbirth and related medical conditions), marital status, registered domestic partner status, sexual orientation, age, ancestry, national origin, veteran status, disability, medical condition, genetic characteristics, and/or any other basis protected by law. This policy covers all facets of employment including, but not limited to: recruitment, selection, placement, promotions, transfers, demotions, terminations, training, and compensation. Mammoth Biosciences requires that all employees be vaccinated against COVID as a condition of at-will employment, with exceptions for medical or religious reasons in compliance with local, state, and federal law. PIf66961b390e2-1932 Required Preferred Job Industries Other
09/05/2025
Full time
Mammoth BioSciences OPPORTUNITY Mammoth Biosciences is seeking a highly motivated Scientist II or Senior Scientist I to join our CMC team in a hands-on, cross-functional role that blends scientific ingenuity with operational excellence. This position is critical for developing, optimizing, scaling, and characterizing LNP-based delivery production processes for Mammoth's cutting-edge gene editing platforms. The ideal candidate will thrive at the interface of process development, analytics, and manufacturingsomeone who can transition smoothly between the bench and discussions with external partners. This is a great opportunity to play a key role in advancing transformative gene editing therapies into the clinic and beyond. KEY RESPONSIBILITIES Design and optimize LNP manufacturing processes by applying Quality by Design (QbD) principles and execute Design of Experiments (DoEs) to identify and control critical process parameters (CPPs) Optimize analytical methods such as DLS, Ribogreen, HPLC-CAD, IP-RP-HPLC, and endotoxin to identify and access critical quality attributes (CQAs) Interpret analytical data to assess process robustness and guide iterative development efforts Collaborate with internal teams to support process improvements and drive innovation Clearly present scientific findings and project updates in internal meetings and to external collaborators Drive technology transfer activities with external CDMO partners by preparing comprehensive technical documentation such as development reports, process summaries, and tech transfer packages to ensure successful external manufacturing Stay current with emerging trends in LNP technologies and contribute to strategic process improvements Track inventory, plan experiments, and contribute to an organized, quality-focused lab environment REQUIRED QUALIFICATIONS Ph.D. in Chemical Engineering, Bioengineering, Pharmaceutical Sciences, Chemistry, or a related field with minimum of 3 years relevant industry experience; or M.S. with 6 years; or B.S. with 8 years Demonstrated expertise in LNP formulation and process development, including hands-on experience with microfluidic and high-shear mixing technologies, tangential flow filtration and dead-end filtration Proficiency in analytical characterization techniques for LNPs, including DLS, fluorescence-based assays and HPLC/UPLC methods (CAD, UV, IP-RP, SEC, IEX), including method development, troubleshooting, and data interpretation Experience with in-process and drug product stability studies Proven success in applying DoEs and statistical analysis tools for process optimization Experience supporting technology transfer of processes to external manufacturing environments, with a strong understanding of tech transfer best practices Demonstrated strong organizational skills A self-starter with demonstrated strong troubleshooting skills, capable of driving projects independently while contributing effectively in a collaborative, multidisciplinary environment PREFERRED QUALIFICATIONS Knowledge of regulatory expectations for CMC development Familiarity with high-throughput screening methods Exposure to cell-based assays for LNP functional characterization Familiarity with data analysis tools and statistical methods such as JMP Experience with AAV vector process development or a strong interest and adaptability to learn viral delivery systems BENEFITS Company-paid health/vision/dental benefits Unlimited vacation and generous sick time Company-sponsored meals and snacks Wellness, caregiver and ergonomics benefits 401(k) with company matching Base Salary Range: $138,000 - $178,000 per year Actual base salary will be determined by relevant professional experience, applicable skills, and internal equity. COMPANY Mammoth Biosciences is a biotechnology company focused on leveraging its proprietary ultracompact CRISPR systems to develop long-term curative therapies, as well as other applications such as decentralized precision diagnostics. Founded by CRISPR pioneer and Nobel laureate Jennifer Doudna and Trevor Martin, Janice Chen, and Lucas Harrington, the companys ultracompact proteins are designed to enable in vivo gene editing in difficult to reach tissues utilizing both nuclease applications and new editing modalities beyond double stranded breaks, including base editing, gene writing, and epigenetic editing. The company is building out its pipeline of potential in vivo gene editing therapeutics and capabilities and has partnerships with leading pharmaceutical and biotechnology companies to broaden the reach of its innovative and proprietary technology. Mammoths deep science and industry experience, along with a robust and differentiated intellectual property portfolio, have enabled the company to further its mission to transform the lives of patients and deliver on the full promise of CRISPR technologies. It is our policy and intent to provide equal opportunity to all persons without regard to race, color, religion, political affiliation, sex/gender (including gender expression/identity, pregnancy, childbirth and related medical conditions), marital status, registered domestic partner status, sexual orientation, age, ancestry, national origin, veteran status, disability, medical condition, genetic characteristics, and/or any other basis protected by law. This policy covers all facets of employment including, but not limited to: recruitment, selection, placement, promotions, transfers, demotions, terminations, training, and compensation. Mammoth Biosciences requires that all employees be vaccinated against COVID as a condition of at-will employment, with exceptions for medical or religious reasons in compliance with local, state, and federal law. PIf66961b390e2-1932 Required Preferred Job Industries Other
Mammoth Biosciences is harnessing the diversity of nature to power the next-generation of CRISPR products. Through the discovery and development of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. By leveraging its internal research and development and exclusive licensing to patents related to Cas12, Cas13, Cas14 and CasPhi, Mammoth Biosciences can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Mammoth is democratizing disease detection with easy and affordable point-of-care tests that allow real-time and simultaneous detection of multiple conditions, along with high-throughput tests that allow for unprecedented testing volume. Further, the company is transforming disease treatment with its proprietary micro-sized CRISPR proteins that enable new editing and delivery options. Based in San Francisco, Mammoth Biosciences is co-founded by CRISPR pioneer Jennifer Doudna and principal founders Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Decheng, Mayfield, NFX, and 8VC, and leading individual investors including Brook Byers, Tim Cook, Bob Nelsen, and Jeff Huber. At Mammoth, we are passionate about transforming lives through innovation. DESCRIPTION Mammoth is currently seeking a talented and detail-oriented Research Associate with experience in molecular biology, flow cytometry, mammalian cell culture, and genetic manipulation of immune cells and/or iPSCs, to join our growing genome editing team. The successful candidate will work closely and collaboratively with colleagues to design/develop, perform and execute experiments on mammalian cell lines and primary human cells to support pipeline projects to develop Cas nucleases for genome editing. They will have the opportunity to contribute broadly across multiple technical areas. The position requires adaptability, excellent planning, strong organizational and communication skills, as well as the ability to work independently in a fast-paced team-oriented and dynamic environment. Responsibilities Execution of molecular biology, cloning, cell culture and a broad range of functional and phenotypic assay workflows to accelerate projects in pre-clinical development. Provide team support for basic laboratory tasks including tissue culture of various cell lines, isolation and culture of primary human cells from whole blood, phenotyping and analytical characterization of cells, genome editing of primary human cells, and virus production Accurately and timely record, document, analyze and communicate laboratory data to senior staff and team members Conducts all activities in a safe and efficient manner, and adheres to site environmental health and safety (EHS) requirements Requirements BS or MS or in molecular and cell biology, Immunology, biochemistry, or a related field, plus at least 3 years relevant laboratory research experience. Industry work experience, especially in immunology/immuno-oncology field, is highly desirable. Proficiency in mammalian cell culture, isolation and culture and genetic manipulation of primary human immune cells.Previous work experience with human NK and T cells and/or CAR-T cell production and characterization is highly desirable. Proficiency with multi-color flow cytometry, FACS sorting and T-cell based assays (T cell phenotyping and memory sub-setting, cytotoxicity, cell proliferation, cytokine secretion). Proficiency in molecular cloning, PCR, qPCR, genomic DNA and mRNA purification, NGS sequencing, ELISA, western blot, retroviral/lentiviral/AAV production, titration, and transduction. Strong organizational and record-keeping skills, excellent interpersonal, verbal and written communication skills. Highly motivated with strong work ethics. Ability to work efficiently and productively in a fast-paced highly collaborative team environment Knowledge of Microsoft Office Suite and other relevant data analysis software (Flowjo, GraphPad Prism, etc.). Benefits Competitive salary Full health/vision/dental benefits Unlimited vacation Free breakfast, lunch, dinner, and barista Free gym Public transportation commuter subsidy 401(k) matching
01/28/2021
Full time
Mammoth Biosciences is harnessing the diversity of nature to power the next-generation of CRISPR products. Through the discovery and development of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. By leveraging its internal research and development and exclusive licensing to patents related to Cas12, Cas13, Cas14 and CasPhi, Mammoth Biosciences can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Mammoth is democratizing disease detection with easy and affordable point-of-care tests that allow real-time and simultaneous detection of multiple conditions, along with high-throughput tests that allow for unprecedented testing volume. Further, the company is transforming disease treatment with its proprietary micro-sized CRISPR proteins that enable new editing and delivery options. Based in San Francisco, Mammoth Biosciences is co-founded by CRISPR pioneer Jennifer Doudna and principal founders Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Decheng, Mayfield, NFX, and 8VC, and leading individual investors including Brook Byers, Tim Cook, Bob Nelsen, and Jeff Huber. At Mammoth, we are passionate about transforming lives through innovation. DESCRIPTION Mammoth is currently seeking a talented and detail-oriented Research Associate with experience in molecular biology, flow cytometry, mammalian cell culture, and genetic manipulation of immune cells and/or iPSCs, to join our growing genome editing team. The successful candidate will work closely and collaboratively with colleagues to design/develop, perform and execute experiments on mammalian cell lines and primary human cells to support pipeline projects to develop Cas nucleases for genome editing. They will have the opportunity to contribute broadly across multiple technical areas. The position requires adaptability, excellent planning, strong organizational and communication skills, as well as the ability to work independently in a fast-paced team-oriented and dynamic environment. Responsibilities Execution of molecular biology, cloning, cell culture and a broad range of functional and phenotypic assay workflows to accelerate projects in pre-clinical development. Provide team support for basic laboratory tasks including tissue culture of various cell lines, isolation and culture of primary human cells from whole blood, phenotyping and analytical characterization of cells, genome editing of primary human cells, and virus production Accurately and timely record, document, analyze and communicate laboratory data to senior staff and team members Conducts all activities in a safe and efficient manner, and adheres to site environmental health and safety (EHS) requirements Requirements BS or MS or in molecular and cell biology, Immunology, biochemistry, or a related field, plus at least 3 years relevant laboratory research experience. Industry work experience, especially in immunology/immuno-oncology field, is highly desirable. Proficiency in mammalian cell culture, isolation and culture and genetic manipulation of primary human immune cells.Previous work experience with human NK and T cells and/or CAR-T cell production and characterization is highly desirable. Proficiency with multi-color flow cytometry, FACS sorting and T-cell based assays (T cell phenotyping and memory sub-setting, cytotoxicity, cell proliferation, cytokine secretion). Proficiency in molecular cloning, PCR, qPCR, genomic DNA and mRNA purification, NGS sequencing, ELISA, western blot, retroviral/lentiviral/AAV production, titration, and transduction. Strong organizational and record-keeping skills, excellent interpersonal, verbal and written communication skills. Highly motivated with strong work ethics. Ability to work efficiently and productively in a fast-paced highly collaborative team environment Knowledge of Microsoft Office Suite and other relevant data analysis software (Flowjo, GraphPad Prism, etc.). Benefits Competitive salary Full health/vision/dental benefits Unlimited vacation Free breakfast, lunch, dinner, and barista Free gym Public transportation commuter subsidy 401(k) matching
COMPANY Mammoth Biosciences is harnessing the diversity of nature to power the next-generation of CRISPR products. Through the discovery and development of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. By leveraging its internal research and development and exclusive licensing to patents related to Cas12, Cas13, Cas14 and CasPhi, Mammoth Biosciences can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Mammoth is democratizing disease detection with easy and affordable point-of-care tests that allow real-time and simultaneous detection of multiple conditions, along with high-throughput tests that allow for unprecedented testing volume. Further, the company is transforming disease treatment with its proprietary micro-sized CRISPR proteins that enable new editing and delivery options. Based in San Francisco, Mammoth Biosciences is co-founded by CRISPR pioneer Jennifer Doudna and principal founders Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Decheng, Mayfield, NFX, and 8VC, and leading individual investors including Brook Byers, Tim Cook, Bob Nelsen, and Jeff Huber. OPPORTUNITY Mammoth is seeking a Research Associate with biochemistry expertise to join its growing Discovery team. The Discovery team is responsible for developing novel CRISPR-associated (Cas) nucleases for genome editing and diagnostic applications. The successful candidate will be responsible for executing laboratory workflows to develop Cas nucleases for genome editing and diagnostic applications. The primary focus for this role will be on protein production and process development. The successful candidate will also have the opportunity to contribute broadly across technical areas, including plasmid construction, NGS assays, and high-throughput screening. This is an excellent development opportunity for researchers who want to learn new skills and start their career working on cutting-edge CRISPR technologies. KEY RESPONSIBILITIES Execution of E. coli-based protein expression and purification workflows Chromatography process development Document and regularly present data at internal meetings Other duties as assigned REQUIRED QUALIFICATIONS Bachelor's degree in molecular biology, biochemistry, or a related field 1 year of laboratory research experience with E. coli-based protein expression systems PREFERRED QUALIFICATIONS Master's degree in molecular biology, biochemistry, or a related field Hands-on experience in the following areas: Protein chromatography methods (ion exchange, affinity, size exclusion) Protein analytical methods (SDS PAGE, BCA Assay, A280, Endotoxin quantification) E. coli transformation and cell culture FPLC Systems Biochemical assays Nucleic acid methods (PCR, DNA/RNA purification and quantitation) Computer and scripting skills (Benchling, Geneious, JMP, SQL, R, python)
01/27/2021
Full time
COMPANY Mammoth Biosciences is harnessing the diversity of nature to power the next-generation of CRISPR products. Through the discovery and development of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. By leveraging its internal research and development and exclusive licensing to patents related to Cas12, Cas13, Cas14 and CasPhi, Mammoth Biosciences can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Mammoth is democratizing disease detection with easy and affordable point-of-care tests that allow real-time and simultaneous detection of multiple conditions, along with high-throughput tests that allow for unprecedented testing volume. Further, the company is transforming disease treatment with its proprietary micro-sized CRISPR proteins that enable new editing and delivery options. Based in San Francisco, Mammoth Biosciences is co-founded by CRISPR pioneer Jennifer Doudna and principal founders Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Decheng, Mayfield, NFX, and 8VC, and leading individual investors including Brook Byers, Tim Cook, Bob Nelsen, and Jeff Huber. OPPORTUNITY Mammoth is seeking a Research Associate with biochemistry expertise to join its growing Discovery team. The Discovery team is responsible for developing novel CRISPR-associated (Cas) nucleases for genome editing and diagnostic applications. The successful candidate will be responsible for executing laboratory workflows to develop Cas nucleases for genome editing and diagnostic applications. The primary focus for this role will be on protein production and process development. The successful candidate will also have the opportunity to contribute broadly across technical areas, including plasmid construction, NGS assays, and high-throughput screening. This is an excellent development opportunity for researchers who want to learn new skills and start their career working on cutting-edge CRISPR technologies. KEY RESPONSIBILITIES Execution of E. coli-based protein expression and purification workflows Chromatography process development Document and regularly present data at internal meetings Other duties as assigned REQUIRED QUALIFICATIONS Bachelor's degree in molecular biology, biochemistry, or a related field 1 year of laboratory research experience with E. coli-based protein expression systems PREFERRED QUALIFICATIONS Master's degree in molecular biology, biochemistry, or a related field Hands-on experience in the following areas: Protein chromatography methods (ion exchange, affinity, size exclusion) Protein analytical methods (SDS PAGE, BCA Assay, A280, Endotoxin quantification) E. coli transformation and cell culture FPLC Systems Biochemical assays Nucleic acid methods (PCR, DNA/RNA purification and quantitation) Computer and scripting skills (Benchling, Geneious, JMP, SQL, R, python)
Mammoth Biosciences is harnessing the diversity of nature to power the next generation of CRISPR products. Through the discovery and development of novel CRISPR products. Through the discovery and development of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. Mammoth aims to democratize disease detection with an easy and affordable point-of-care test that allows real-time and simultaneous detection of multiple conditions. By leveraging its internal research and development and exclusive licensing to Cas12, Cas13 and Cas14, Mammoth can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Based in San Francisco, Mammoth Biosciences is co-founded by CRISPR pioneer Jennifer Doudna and principal founders Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Mayfield, NFX, and 8VC, Decheng and leading individual investors including Brook Byers, Tim Cook, and Jeff Huber. DESCRIPTION You get excited about a company and their mission...you have a mammoth amount to offer...but you don't see the role for you on our careers page. We want to hear from you and see if there is a spot for you on our team. Please apply under this role and we will look at your resume to see if there is a fit now or in the future.
01/15/2021
Full time
Mammoth Biosciences is harnessing the diversity of nature to power the next generation of CRISPR products. Through the discovery and development of novel CRISPR products. Through the discovery and development of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. Mammoth aims to democratize disease detection with an easy and affordable point-of-care test that allows real-time and simultaneous detection of multiple conditions. By leveraging its internal research and development and exclusive licensing to Cas12, Cas13 and Cas14, Mammoth can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Based in San Francisco, Mammoth Biosciences is co-founded by CRISPR pioneer Jennifer Doudna and principal founders Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Mayfield, NFX, and 8VC, Decheng and leading individual investors including Brook Byers, Tim Cook, and Jeff Huber. DESCRIPTION You get excited about a company and their mission...you have a mammoth amount to offer...but you don't see the role for you on our careers page. We want to hear from you and see if there is a spot for you on our team. Please apply under this role and we will look at your resume to see if there is a fit now or in the future.