George Washington University
Washington, Washington DC
I. DEPARTMENT INFORMATION Job Description Summary: GW has everything you need to succeed in discovery. Outstanding research , state-of-the-art facilities and world-class faculty support discovery and innovative solutions. Researchers and faculty across campus advance interdisciplinary and emerging research and scholarship. Our active GW Postdoctoral Association welcomes your engagement in social and professional activities as you join our research teams. Research guides, coding and data management solutions as well as extensive online journals from GW Libraries help to guide your study. The Office of the Vice Provost for Research (OVPR) supports the research infrastructure and facilitates several institutional research initiatives . The Department of Pathology at the George Washington University School of Medicine and Health Sciences is a dynamic department in a world-class medical school located in the heart of the nation's capital, Washington, DC. The talented and dedicated faculty, fellows, residents, students, and staff combine to bring the very best in pathology and clinical laboratory services, medical education, and research to our community, the region, and the nation. The Lab is focused on studies of Genomic and Molecular Mechanisms of Esophageal & Gastrointestinal Cancer and Pre-Cancer and Biomarkers of Digestive Organ Cancers. We use mouse models, ex vivo 3D organoid models and human tissues. Mouse models are central to our in vivo studies. The Postdoctoral Associate position is primarily a temporary/training position in which the incumbent plays a substantive role in planning and conducting research by designing and conducting experiments in a controlled laboratory setting. In collaboration with the Principal Investigator, this role will participate in the planning of independent research, will analyze and interpret data, will publish results, will represent the university at conferences and meetings, and may develop new theories and methodologies. This position may also help the Principal Investigator to lead and direct the work of work under the supervision of experienced researchers. We are seeking a highly motivated individual to join a research team working together to understand the molecular mechanisms of pre-cancer progression to cancer and biomarkers for early interception and therapies of gastrointestinal and digestive organ cancers. Mouse and organoid models are central to our studies. This position involves overseeing and performing mouse colony maintenance, genotyping, tissue collection from animals, performing experiments and assays including genomic and transcriptomic analyses, development and optimization of assays, writing protocols, data analysis and presentation at lab meetings/conferences, writing reports, preparation of manuscripts for peer review and publications in professional journals, participation in an extramurally funded research program working with the PI on NIH and other extramural grants. Postdocs will design, organize and conduct specialized and advanced experiments using established scientific protocols, troubleshoot and design new protocols, summarize findings and publish results in research journals, under the general supervision of a faculty member. In some cases, postdocs will assume general responsibility for general scientific operations in the lab, and/or provide supervision and guidance to laboratory staff, trainees and other researchers. This role performs work under the supervision of experienced researchers in the Sepulveda laboratory. Additional duties include: Performing experiments and assays including spatial transcriptomics of pre-cancer and cancer lesions; studies of pre-cancer and cancer immune microenvironment. Computational and AI methods for digital imaging. Studies of pre-cancer and cancer immune microenvironment. Working with and supervising students in the laboratory. This position performs other duties as assigned. The omission of specific duties does not preclude the supervisor from assigning duties that are logically related to the position. A Postdoctoral Associate: holds a PhD or equivalent degree; develops skills by participating in scholarly projects that are actively mentored by a member of the university faculty; receives financial support in the form of a salary that is typically funded by grants, contracts or departmental funds secured by a faculty mentor; and has a limited term of up to five years at GW. In collaboration with experienced researchers, this role will participate in the planning and conduction of discovery research, data analysis and interpretation, preparation of materials for publication, presentations at conferences and meetings, and may develop new theories and methodologies. This role can expect timely advice as to research directions and career planning for a future independent career. Performs other related duties as assigned. The omission of specific duties does not preclude the supervisor from assigning duties that are logically related to the position. Special Instructions to the Applicant: Appointments are limited-term and are designed for those who have recently received a doctorate. Appointments are made to academic departments with the approval of the department head and may require senior officer approval. Appointments are usually for not less than one term and are normally not renewed beyond a total of five years. This appointment is not intended for long-term, indefinite, or career appointments. Minimum Qualifications: Qualified candidates will hold a Doctoral degree or comparable doctoral degree in a related discipline. Degree must be conferred by the start date of the position. Additional Required Licenses/Certifications/Posting Specific Minimum Qualifications: Preferred Qualifications: Doctoral degree in Molecular Biology /Cell Biology/Genomics/Computational Biology or a related discipline with experience in molecular biology techniques and mouse models preferred. Typical Hiring Range Commensurate with Experience II. POSITION INFORMATION Campus Location: Foggy Bottom, Washington, D.C. College/School/Department: School of Medicine and Health Sciences (SMHS) Family Research and Labs Sub-Family Laboratory Research Stream Individual Contributor Level Level 3 Full-Time/Part-Time: Full time -> FTE 1.00 Hours Per Week: 40 Work Schedule: Monday through Friday, 8:30 - 5:00. Will this job require the employee to work on site? Yes Employee Onsite Status On-campus (in person) Telework: Required Background Check Criminal History Screening, Education/Degree/Certifications Verification, Social Security Number Trace, and Sex Offender Registry Search Special Instructions to Applicants: Employer will not sponsor for employment Visa status Internal Applicants Only? No Posting Number: R001797 Job Open Date: 12/15/2022 Job Close Date: If temporary, grant funded or limited term appointment, position funded until: Background Screening Successful Completion of a Background Screening will be required as a condition of hire. EEO Statement: The university is an Equal Employment Opportunity/Affirmative Action employer that does not unlawfully discriminate in any of its programs or activities on the basis of race, color, religion, sex, national origin, age, disability, veteran status, sexual orientation, gender identity or expression, or on any other basis prohibited by applicable law.
03/09/2024
Full time
I. DEPARTMENT INFORMATION Job Description Summary: GW has everything you need to succeed in discovery. Outstanding research , state-of-the-art facilities and world-class faculty support discovery and innovative solutions. Researchers and faculty across campus advance interdisciplinary and emerging research and scholarship. Our active GW Postdoctoral Association welcomes your engagement in social and professional activities as you join our research teams. Research guides, coding and data management solutions as well as extensive online journals from GW Libraries help to guide your study. The Office of the Vice Provost for Research (OVPR) supports the research infrastructure and facilitates several institutional research initiatives . The Department of Pathology at the George Washington University School of Medicine and Health Sciences is a dynamic department in a world-class medical school located in the heart of the nation's capital, Washington, DC. The talented and dedicated faculty, fellows, residents, students, and staff combine to bring the very best in pathology and clinical laboratory services, medical education, and research to our community, the region, and the nation. The Lab is focused on studies of Genomic and Molecular Mechanisms of Esophageal & Gastrointestinal Cancer and Pre-Cancer and Biomarkers of Digestive Organ Cancers. We use mouse models, ex vivo 3D organoid models and human tissues. Mouse models are central to our in vivo studies. The Postdoctoral Associate position is primarily a temporary/training position in which the incumbent plays a substantive role in planning and conducting research by designing and conducting experiments in a controlled laboratory setting. In collaboration with the Principal Investigator, this role will participate in the planning of independent research, will analyze and interpret data, will publish results, will represent the university at conferences and meetings, and may develop new theories and methodologies. This position may also help the Principal Investigator to lead and direct the work of work under the supervision of experienced researchers. We are seeking a highly motivated individual to join a research team working together to understand the molecular mechanisms of pre-cancer progression to cancer and biomarkers for early interception and therapies of gastrointestinal and digestive organ cancers. Mouse and organoid models are central to our studies. This position involves overseeing and performing mouse colony maintenance, genotyping, tissue collection from animals, performing experiments and assays including genomic and transcriptomic analyses, development and optimization of assays, writing protocols, data analysis and presentation at lab meetings/conferences, writing reports, preparation of manuscripts for peer review and publications in professional journals, participation in an extramurally funded research program working with the PI on NIH and other extramural grants. Postdocs will design, organize and conduct specialized and advanced experiments using established scientific protocols, troubleshoot and design new protocols, summarize findings and publish results in research journals, under the general supervision of a faculty member. In some cases, postdocs will assume general responsibility for general scientific operations in the lab, and/or provide supervision and guidance to laboratory staff, trainees and other researchers. This role performs work under the supervision of experienced researchers in the Sepulveda laboratory. Additional duties include: Performing experiments and assays including spatial transcriptomics of pre-cancer and cancer lesions; studies of pre-cancer and cancer immune microenvironment. Computational and AI methods for digital imaging. Studies of pre-cancer and cancer immune microenvironment. Working with and supervising students in the laboratory. This position performs other duties as assigned. The omission of specific duties does not preclude the supervisor from assigning duties that are logically related to the position. A Postdoctoral Associate: holds a PhD or equivalent degree; develops skills by participating in scholarly projects that are actively mentored by a member of the university faculty; receives financial support in the form of a salary that is typically funded by grants, contracts or departmental funds secured by a faculty mentor; and has a limited term of up to five years at GW. In collaboration with experienced researchers, this role will participate in the planning and conduction of discovery research, data analysis and interpretation, preparation of materials for publication, presentations at conferences and meetings, and may develop new theories and methodologies. This role can expect timely advice as to research directions and career planning for a future independent career. Performs other related duties as assigned. The omission of specific duties does not preclude the supervisor from assigning duties that are logically related to the position. Special Instructions to the Applicant: Appointments are limited-term and are designed for those who have recently received a doctorate. Appointments are made to academic departments with the approval of the department head and may require senior officer approval. Appointments are usually for not less than one term and are normally not renewed beyond a total of five years. This appointment is not intended for long-term, indefinite, or career appointments. Minimum Qualifications: Qualified candidates will hold a Doctoral degree or comparable doctoral degree in a related discipline. Degree must be conferred by the start date of the position. Additional Required Licenses/Certifications/Posting Specific Minimum Qualifications: Preferred Qualifications: Doctoral degree in Molecular Biology /Cell Biology/Genomics/Computational Biology or a related discipline with experience in molecular biology techniques and mouse models preferred. Typical Hiring Range Commensurate with Experience II. POSITION INFORMATION Campus Location: Foggy Bottom, Washington, D.C. College/School/Department: School of Medicine and Health Sciences (SMHS) Family Research and Labs Sub-Family Laboratory Research Stream Individual Contributor Level Level 3 Full-Time/Part-Time: Full time -> FTE 1.00 Hours Per Week: 40 Work Schedule: Monday through Friday, 8:30 - 5:00. Will this job require the employee to work on site? Yes Employee Onsite Status On-campus (in person) Telework: Required Background Check Criminal History Screening, Education/Degree/Certifications Verification, Social Security Number Trace, and Sex Offender Registry Search Special Instructions to Applicants: Employer will not sponsor for employment Visa status Internal Applicants Only? No Posting Number: R001797 Job Open Date: 12/15/2022 Job Close Date: If temporary, grant funded or limited term appointment, position funded until: Background Screening Successful Completion of a Background Screening will be required as a condition of hire. EEO Statement: The university is an Equal Employment Opportunity/Affirmative Action employer that does not unlawfully discriminate in any of its programs or activities on the basis of race, color, religion, sex, national origin, age, disability, veteran status, sexual orientation, gender identity or expression, or on any other basis prohibited by applicable law.
George Washington University
Washington, Washington DC
I. DEPARTMENT INFORMATION Job Description Summary: Founded in 1824, the GW School of Medicine and Health Sciences (SMHS) is the 11th oldest medical school in the country and the first in the nation's capital. Since its establishment, the school has been at the forefront of medical education and has grown to include highly ranked programs in the health and biomedical sciences. With dozens of top-tier residency programs, SMHS is a competitive and esteemed destination for medical school graduates across the country. The group's main interests are advancing our understanding of the molecular and cellular pathological mechanisms of myasthenia gravis using innovative rodent models and cutting-edge technologies and developing antigen-specific immunosuppressive therapies for the disease. The research is highly interdisciplinary, relying on tools from molecular biology, biochemistry, structural biology, AI-based structure prediction, transgenic models, immunology, and neuroscience, but also including industrial collaboration. The laboratory of Dr. Jie Luo is seeking an experienced candidate at the rank of Senior Research Associate. This position will independently carry out research/development activities by designing, organizing, and conducting experiments, troubleshoot, prepare technical reports and documentation of outcomes, and assist with the preparation of results for publication, under the general supervision of the Principal Investigator. This experienced researcher carries out research/development activities by using quantitative and qualitative methods to collect, analyze and report data activities. Using a wide degree of creativity and latitude, the core responsibilities of this position are to make detailed observations, analyze data, and interpret results, with limited guidance from the Principal Investigator. This position will assume general responsibility for general scientific operations in the lab, and/or provide guidance to junior staff, graduate and undergraduate student. This role is familiar with a variety of field concepts, practices, and procedures, and can perform a variety of research tasks. This position typically reports to a project supervisor or project manager and may provide guide to lower-level staff. Duties Include: Perform injection, tissue collection, clinical evaluation, recording and analyzing of results in animal experiments Perform immunoassays, flow cytometry analysis, mammalian and bacterial cell cultures, DNA preparation, and protein purification. Plan and schedule experiments consistent with the timeline and objectives of ongoing research projects. Support faculty and staff in the research group, as well as external partners, in communicating results, figures, charts, and tables as needed Prepare with the Principal Investigator regulatory documents, manuscripts, and grants. Work collaboratively with multi-disciplinary teams Compile results and prepare technical reports and documentation of outcomes. Perform other related duties as assigned. The omission of specific duties does not preclude the supervisor from assigning duties that are logically related to the position Minimum Qualifications: Qualified candidates will hold a master's degree and 3 years of direct experience in a related discipline. Degree must be conferred by the start date of the position Additional Required Licenses/Certifications/Posting Specific Minimum Qualifications: Preferred Qualifications: Prior experience in molecular biological techniques, cell culture, and animal models is essential; additional experience in flow cytometry, immunological and biochemical techniques is a plus. Experience in the preparation of scientific publications and presentations Typical Hiring Range Commensurate with Experience II. POSITION INFORMATION Campus Location: Foggy Bottom, Washington, D.C. College/School/Department: School of Medicine and Health Sciences (SMHS) Family Research and Labs Sub-Family Laboratory Research Stream Individual Contributor Level Level 2 Full-Time/Part-Time: Full time -> FTE 1.00 Hours Per Week: 40 Work Schedule: Monday through Friday, 8:30am-5:00pm Will this job require the employee to work on site? Yes Employee Onsite Status On-campus (in person) Telework: Required Background Check Criminal History Screening, Education/Degree/Certifications Verification, Social Security Number Trace, and Sex Offender Registry Search Special Instructions to Applicants: Employer will not sponsor for employment Visa status Internal Applicants Only? No Posting Number: R001824 Job Open Date: 01/23/2023 Job Close Date: If temporary, grant funded or limited term appointment, position funded until: Background Screening Successful Completion of a Background Screening will be required as a condition of hire. EEO Statement: The university is an Equal Employment Opportunity/Affirmative Action employer that does not unlawfully discriminate in any of its programs or activities on the basis of race, color, religion, sex, national origin, age, disability, veteran status, sexual orientation, gender identity or expression, or on any other basis prohibited by applicable law.
03/09/2024
Full time
I. DEPARTMENT INFORMATION Job Description Summary: Founded in 1824, the GW School of Medicine and Health Sciences (SMHS) is the 11th oldest medical school in the country and the first in the nation's capital. Since its establishment, the school has been at the forefront of medical education and has grown to include highly ranked programs in the health and biomedical sciences. With dozens of top-tier residency programs, SMHS is a competitive and esteemed destination for medical school graduates across the country. The group's main interests are advancing our understanding of the molecular and cellular pathological mechanisms of myasthenia gravis using innovative rodent models and cutting-edge technologies and developing antigen-specific immunosuppressive therapies for the disease. The research is highly interdisciplinary, relying on tools from molecular biology, biochemistry, structural biology, AI-based structure prediction, transgenic models, immunology, and neuroscience, but also including industrial collaboration. The laboratory of Dr. Jie Luo is seeking an experienced candidate at the rank of Senior Research Associate. This position will independently carry out research/development activities by designing, organizing, and conducting experiments, troubleshoot, prepare technical reports and documentation of outcomes, and assist with the preparation of results for publication, under the general supervision of the Principal Investigator. This experienced researcher carries out research/development activities by using quantitative and qualitative methods to collect, analyze and report data activities. Using a wide degree of creativity and latitude, the core responsibilities of this position are to make detailed observations, analyze data, and interpret results, with limited guidance from the Principal Investigator. This position will assume general responsibility for general scientific operations in the lab, and/or provide guidance to junior staff, graduate and undergraduate student. This role is familiar with a variety of field concepts, practices, and procedures, and can perform a variety of research tasks. This position typically reports to a project supervisor or project manager and may provide guide to lower-level staff. Duties Include: Perform injection, tissue collection, clinical evaluation, recording and analyzing of results in animal experiments Perform immunoassays, flow cytometry analysis, mammalian and bacterial cell cultures, DNA preparation, and protein purification. Plan and schedule experiments consistent with the timeline and objectives of ongoing research projects. Support faculty and staff in the research group, as well as external partners, in communicating results, figures, charts, and tables as needed Prepare with the Principal Investigator regulatory documents, manuscripts, and grants. Work collaboratively with multi-disciplinary teams Compile results and prepare technical reports and documentation of outcomes. Perform other related duties as assigned. The omission of specific duties does not preclude the supervisor from assigning duties that are logically related to the position Minimum Qualifications: Qualified candidates will hold a master's degree and 3 years of direct experience in a related discipline. Degree must be conferred by the start date of the position Additional Required Licenses/Certifications/Posting Specific Minimum Qualifications: Preferred Qualifications: Prior experience in molecular biological techniques, cell culture, and animal models is essential; additional experience in flow cytometry, immunological and biochemical techniques is a plus. Experience in the preparation of scientific publications and presentations Typical Hiring Range Commensurate with Experience II. POSITION INFORMATION Campus Location: Foggy Bottom, Washington, D.C. College/School/Department: School of Medicine and Health Sciences (SMHS) Family Research and Labs Sub-Family Laboratory Research Stream Individual Contributor Level Level 2 Full-Time/Part-Time: Full time -> FTE 1.00 Hours Per Week: 40 Work Schedule: Monday through Friday, 8:30am-5:00pm Will this job require the employee to work on site? Yes Employee Onsite Status On-campus (in person) Telework: Required Background Check Criminal History Screening, Education/Degree/Certifications Verification, Social Security Number Trace, and Sex Offender Registry Search Special Instructions to Applicants: Employer will not sponsor for employment Visa status Internal Applicants Only? No Posting Number: R001824 Job Open Date: 01/23/2023 Job Close Date: If temporary, grant funded or limited term appointment, position funded until: Background Screening Successful Completion of a Background Screening will be required as a condition of hire. EEO Statement: The university is an Equal Employment Opportunity/Affirmative Action employer that does not unlawfully discriminate in any of its programs or activities on the basis of race, color, religion, sex, national origin, age, disability, veteran status, sexual orientation, gender identity or expression, or on any other basis prohibited by applicable law.
Obsidian Therapeutics is pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. Obsidian's programs apply our CytoDriveTM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety. Our Opportunity… We're looking for a highly motivated Senior Research Associate with expertise in cell culture and upstream process development to contribute to developing and characterizing manufacturing processes for multiple cell therapy products in our pipeline targeting cancer. You will collaborate with the research groups to transfer and build on foundational know-how to deliver phase-appropriate manufacturing processes that meets the quality target profile. As part of our Technical Development and Manufacturing department, you will be a vital part of a highly collaborative team working to achieve Obsidian's vision by translating innovative science into medical breakthroughs for patients. This is a unique opportunity to be a scientific contributor in a well-funded early-stage company with blue-chip investors, field-leading advisors, and a highly experienced team. You will help us build a dynamic, innovative, passionate, collaborative, and successful organization focused on delivering transformative therapies in areas of greatest clinical need. Located in the heart of Cambridge, we're proud of our diverse talented team and committed to cultivating an environment of inclusion where each person feels a strong sense of belonging and is supported to continuously learn and contribute their best work. We offer competitive salary and benefits, and potential for employee ownership through stock options. You Will… Plan and execute process development experiments with engineered T-cell and other immune cell product candidates to define process, optimize and scale-up for clinical manufacturing encompassing all steps of the process including starting material isolation, expansion, viral transduction, and cryopreservation Perform in-process assays to measure cell number and characterize product using flow cytometry and other techniques Interpret results, troubleshoot technical hurdles, and propose solutions to the team Maintain excellent records of experiments in lab notebooks in a timely manner Collaborate effectively across Obsidian organization, present internally to project teams, management, and scientific teams Author and review technical reports detailing experimental work and summarizing results Maintain a safe work environment in accordance with policies/procedures/regulations Maintain cell cultures and cell banks as needed to support experimental work including occasional production of viral vectors Support lab functions-including, but not limited to restocking consumables, maintaining equipment and cleaning You Bring… Core Qualifications BS or MS degree in chemical engineering, biochemical engineering, biology or relevant field, with 2-6 years of experience in biotech/pharma setting Strong experience with cell culture techniques with both adherent and suspension cell lines Hands-on experience with plasmid transfection and viral transduction using lentiviral/retroviral vectors Experience with analytical methods for cell characterization such as flow cytometry, quantitative PCR, western blot, and ELISA Strong troubleshooting and communication skills Strong record-keeping and organization skills, with an ability to manage multiple tasks in parallel Highly collaborative work style Ability to work independently and as part of teams to meet deadlines in a fast-paced environment, prioritize work across multiple projects, and adapt to changes in order to fulfill our mission Bonus Qualifications Experience with T-cell (or other immune cell) selection, viral transduction, gene editing technologies, cell expansion and cryopreservation Basic knowledge of immunology and experience working with immune system cells Experience with design of experiment (DOE) approaches and statistical analysis of data Experience with the culture of mammalian cells in small -scale and large-scale bioreactor systems and associated control strategies and automation Prior experience working with CDMOs, technology transfer in the context of process development to support GMP manufacturing
03/23/2021
Full time
Obsidian Therapeutics is pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. Obsidian's programs apply our CytoDriveTM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety. Our Opportunity… We're looking for a highly motivated Senior Research Associate with expertise in cell culture and upstream process development to contribute to developing and characterizing manufacturing processes for multiple cell therapy products in our pipeline targeting cancer. You will collaborate with the research groups to transfer and build on foundational know-how to deliver phase-appropriate manufacturing processes that meets the quality target profile. As part of our Technical Development and Manufacturing department, you will be a vital part of a highly collaborative team working to achieve Obsidian's vision by translating innovative science into medical breakthroughs for patients. This is a unique opportunity to be a scientific contributor in a well-funded early-stage company with blue-chip investors, field-leading advisors, and a highly experienced team. You will help us build a dynamic, innovative, passionate, collaborative, and successful organization focused on delivering transformative therapies in areas of greatest clinical need. Located in the heart of Cambridge, we're proud of our diverse talented team and committed to cultivating an environment of inclusion where each person feels a strong sense of belonging and is supported to continuously learn and contribute their best work. We offer competitive salary and benefits, and potential for employee ownership through stock options. You Will… Plan and execute process development experiments with engineered T-cell and other immune cell product candidates to define process, optimize and scale-up for clinical manufacturing encompassing all steps of the process including starting material isolation, expansion, viral transduction, and cryopreservation Perform in-process assays to measure cell number and characterize product using flow cytometry and other techniques Interpret results, troubleshoot technical hurdles, and propose solutions to the team Maintain excellent records of experiments in lab notebooks in a timely manner Collaborate effectively across Obsidian organization, present internally to project teams, management, and scientific teams Author and review technical reports detailing experimental work and summarizing results Maintain a safe work environment in accordance with policies/procedures/regulations Maintain cell cultures and cell banks as needed to support experimental work including occasional production of viral vectors Support lab functions-including, but not limited to restocking consumables, maintaining equipment and cleaning You Bring… Core Qualifications BS or MS degree in chemical engineering, biochemical engineering, biology or relevant field, with 2-6 years of experience in biotech/pharma setting Strong experience with cell culture techniques with both adherent and suspension cell lines Hands-on experience with plasmid transfection and viral transduction using lentiviral/retroviral vectors Experience with analytical methods for cell characterization such as flow cytometry, quantitative PCR, western blot, and ELISA Strong troubleshooting and communication skills Strong record-keeping and organization skills, with an ability to manage multiple tasks in parallel Highly collaborative work style Ability to work independently and as part of teams to meet deadlines in a fast-paced environment, prioritize work across multiple projects, and adapt to changes in order to fulfill our mission Bonus Qualifications Experience with T-cell (or other immune cell) selection, viral transduction, gene editing technologies, cell expansion and cryopreservation Basic knowledge of immunology and experience working with immune system cells Experience with design of experiment (DOE) approaches and statistical analysis of data Experience with the culture of mammalian cells in small -scale and large-scale bioreactor systems and associated control strategies and automation Prior experience working with CDMOs, technology transfer in the context of process development to support GMP manufacturing
Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. Obsidian's programs apply our CytoDrive TM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety. Our Opportunity… We're looking for a scientist with a strong background in analytical development to contribute to building Obsidian's analytical sciences capabilities for process and product characterization, contributing to the first autologous cell therapy product in our pipeline targeting cancer. In this role, you'll work collaboratively with the Manufacturing, Process Development, Regulatory, and Quality functions to develop analytical tools for the characterization of viral vectors and cell therapy products to support our clinical pipeline. As part of our Technical Development and Manufacturing department, you'll be a vital part of a highly collaborative team working to achieve Obsidian's vision by translating innovative science into medical breakthroughs for patients. This is a unique opportunity in a well-funded early-stage company with blue chip investors, field-leading advisors, and a highly experienced team. You will help us build a dynamic, innovative, passionate, collaborative, and successful organization focused on delivering transformative therapies in areas of greatest clinical need. Located in the heart of Cambridge, we're proud of our diverse talented team and committed to cultivating an environment of inclusion where each person feels a strong sense of belonging and is supported to continuously learn and contribute their best work. We offer competitive salary and benefits, and potential for employee ownership through stock options. Apply to . You Will… Apply your expertise in developing and qualifying analytical assays to support the release and characterization of viral vector and cell therapy products in early and later phases of development Design, plan, and execute experiments in Analytical Development, working both independently and as part of a multidisciplinary team of researchers Support development and/or improvement of methods in collaboration with research and vector process development to characterize viral vector production methods and primary cell transduction (eg T cell, TIL, NK cell) Support introduction of new analytical technologies, implementation of common reagents and controls, trending of data, and protocols to support construct screening, research studies, research vector production, and CDMO tech transfer activities Interpret results, troubleshoot technical hurdles, and propose solutions to the team Maintain excellent records of experiments, including Electronic Notebook entries, authoring of protocols, test methods, and study reports Collaborate effectively across Obsidian organization, present internally to project teams, management, and scientific teams You Bring… Core Qualifications BS or MS in a relevant discipline (molecular biology, cell biology, biomolecular engineering, biotechnology or relevant field) Minimum of 6-10 years relevant industry experience including prior direct experience with analysis of viral vectors and/or cell therapies Demonstrated track record with analytical techniques focused on cell biology, immunology, molecular biology, and potency assay development Subject matter expertise in analytical development, including methods such as multicolor flow cytometry/ FACS , cell line/ primary cell transduction/ transfection, , qPCR/ ddPCR, ELISA, MSD, and western blot Strong troubleshooting and communication skills. Highly organized with strong record keeping and an ability to manage multiple tasks in parallel Highly collaborative working style and ability to adapt in a fast paced, rapidly developing environment Ability to work with general direction to meet objectives and timelines, adapting to changes and priorities in order to fulfill our mission Bonus Qualifications Experience with development of flow cytometry-based assays for release and characterization Experience in analytical development supporting early and/ or late stage development of biologics is highly desirable (cell therapy, gene therapy, vaccines, antibodies) Demonstrated track record with analytical development and method qualification for characterization of engineered cell therapies Familiarity with viral vector production and cell line engineering Experience with Design of Experiment (DOE) approaches and application to analytical development Experience working with CDMOs/ CTLs for method transfer/ development/ qualification/ validation Familiarity with FDA and EMA guidance documents relevant to gene and cell therapy analytical development and method validation
03/23/2021
Full time
Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. Obsidian's programs apply our CytoDrive TM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety. Our Opportunity… We're looking for a scientist with a strong background in analytical development to contribute to building Obsidian's analytical sciences capabilities for process and product characterization, contributing to the first autologous cell therapy product in our pipeline targeting cancer. In this role, you'll work collaboratively with the Manufacturing, Process Development, Regulatory, and Quality functions to develop analytical tools for the characterization of viral vectors and cell therapy products to support our clinical pipeline. As part of our Technical Development and Manufacturing department, you'll be a vital part of a highly collaborative team working to achieve Obsidian's vision by translating innovative science into medical breakthroughs for patients. This is a unique opportunity in a well-funded early-stage company with blue chip investors, field-leading advisors, and a highly experienced team. You will help us build a dynamic, innovative, passionate, collaborative, and successful organization focused on delivering transformative therapies in areas of greatest clinical need. Located in the heart of Cambridge, we're proud of our diverse talented team and committed to cultivating an environment of inclusion where each person feels a strong sense of belonging and is supported to continuously learn and contribute their best work. We offer competitive salary and benefits, and potential for employee ownership through stock options. Apply to . You Will… Apply your expertise in developing and qualifying analytical assays to support the release and characterization of viral vector and cell therapy products in early and later phases of development Design, plan, and execute experiments in Analytical Development, working both independently and as part of a multidisciplinary team of researchers Support development and/or improvement of methods in collaboration with research and vector process development to characterize viral vector production methods and primary cell transduction (eg T cell, TIL, NK cell) Support introduction of new analytical technologies, implementation of common reagents and controls, trending of data, and protocols to support construct screening, research studies, research vector production, and CDMO tech transfer activities Interpret results, troubleshoot technical hurdles, and propose solutions to the team Maintain excellent records of experiments, including Electronic Notebook entries, authoring of protocols, test methods, and study reports Collaborate effectively across Obsidian organization, present internally to project teams, management, and scientific teams You Bring… Core Qualifications BS or MS in a relevant discipline (molecular biology, cell biology, biomolecular engineering, biotechnology or relevant field) Minimum of 6-10 years relevant industry experience including prior direct experience with analysis of viral vectors and/or cell therapies Demonstrated track record with analytical techniques focused on cell biology, immunology, molecular biology, and potency assay development Subject matter expertise in analytical development, including methods such as multicolor flow cytometry/ FACS , cell line/ primary cell transduction/ transfection, , qPCR/ ddPCR, ELISA, MSD, and western blot Strong troubleshooting and communication skills. Highly organized with strong record keeping and an ability to manage multiple tasks in parallel Highly collaborative working style and ability to adapt in a fast paced, rapidly developing environment Ability to work with general direction to meet objectives and timelines, adapting to changes and priorities in order to fulfill our mission Bonus Qualifications Experience with development of flow cytometry-based assays for release and characterization Experience in analytical development supporting early and/ or late stage development of biologics is highly desirable (cell therapy, gene therapy, vaccines, antibodies) Demonstrated track record with analytical development and method qualification for characterization of engineered cell therapies Familiarity with viral vector production and cell line engineering Experience with Design of Experiment (DOE) approaches and application to analytical development Experience working with CDMOs/ CTLs for method transfer/ development/ qualification/ validation Familiarity with FDA and EMA guidance documents relevant to gene and cell therapy analytical development and method validation
We Are Genetic Navigators bringing together passionate, creative and dedicated professionals to join a rapidly-growing startup on our mission to translate novel genetic insights into lifesaving medicines. We are integrating human genetics and functional genomics to decode the mysteries of genetic modifiers, leading us to new medicines we'll develop for a range of severe diseases. We are seeking an exceptional, hands-on researcher to join our functional genomics team and contribute to advancing our functional screening for genetic modifiers. The successful candidate will work with Senior Scientist to execute cellular assays and engineer cell models for relevant disease indications. As this team is at the core of our early research, the successful candidate will collaborate with multi-disciplinary scientists for target discovery, being a key contributor generating functional genomics data. The role is hands on in nature and requires a detail-oriented mindset and a curious, motivated disposition to accomplish experiments. Your Role in Navigating The Maze: Design, optimize, and execute cellular assays to enable high throughput functional genomics screening aimed at identifying novel genetic modifier targets. Engineer cell models in disease-relevant systems for hit validation Perform mechanism of action studies, including analysis of protein levels, gene expression, and pathway activity to validate and advance functional genomics targets. Collaborate closely with colleagues on our therapeutic area teams to develop assay strategies for primary screens and secondary assays. Independently summarize, analyze, and regularly present work internally and externally. Contribute to development of automated processes, data analysis methods, and advanced assay technologies. Your Navigation Tools: BS/MS in a Biology related field and at least 3-10 years of relevant experience Broad laboratory experience and skills in molecular and cell biology (DNA/RNA extraction, PCR/qPCR, FACs, NGS library prep, etc.) Expertise in cell-based assay development using a variety of readout technologies such as luciferase, ELISA/immuno-assays, and qPCR/ddPCR gene expression-based assays Expertise in mammalian cell culture is required; experience with genetic perturbation of mammalian cells (CRISPR, RNAi, ASO, lentiviral cell engineering, etc.) is strongly preferred Experience with arrayed screening and comfort with laboratory automation is preferred. Experience with iPSC-derived cell models is a plus Strong experimental design, troubleshooting, and data analysis skills Excellent communication, presentation, collaboration, and organizational skills to work as part of a team. Proven ability to think critically, adapt to new data, multi-task, and work in a fast-paced environment
03/05/2021
Full time
We Are Genetic Navigators bringing together passionate, creative and dedicated professionals to join a rapidly-growing startup on our mission to translate novel genetic insights into lifesaving medicines. We are integrating human genetics and functional genomics to decode the mysteries of genetic modifiers, leading us to new medicines we'll develop for a range of severe diseases. We are seeking an exceptional, hands-on researcher to join our functional genomics team and contribute to advancing our functional screening for genetic modifiers. The successful candidate will work with Senior Scientist to execute cellular assays and engineer cell models for relevant disease indications. As this team is at the core of our early research, the successful candidate will collaborate with multi-disciplinary scientists for target discovery, being a key contributor generating functional genomics data. The role is hands on in nature and requires a detail-oriented mindset and a curious, motivated disposition to accomplish experiments. Your Role in Navigating The Maze: Design, optimize, and execute cellular assays to enable high throughput functional genomics screening aimed at identifying novel genetic modifier targets. Engineer cell models in disease-relevant systems for hit validation Perform mechanism of action studies, including analysis of protein levels, gene expression, and pathway activity to validate and advance functional genomics targets. Collaborate closely with colleagues on our therapeutic area teams to develop assay strategies for primary screens and secondary assays. Independently summarize, analyze, and regularly present work internally and externally. Contribute to development of automated processes, data analysis methods, and advanced assay technologies. Your Navigation Tools: BS/MS in a Biology related field and at least 3-10 years of relevant experience Broad laboratory experience and skills in molecular and cell biology (DNA/RNA extraction, PCR/qPCR, FACs, NGS library prep, etc.) Expertise in cell-based assay development using a variety of readout technologies such as luciferase, ELISA/immuno-assays, and qPCR/ddPCR gene expression-based assays Expertise in mammalian cell culture is required; experience with genetic perturbation of mammalian cells (CRISPR, RNAi, ASO, lentiviral cell engineering, etc.) is strongly preferred Experience with arrayed screening and comfort with laboratory automation is preferred. Experience with iPSC-derived cell models is a plus Strong experimental design, troubleshooting, and data analysis skills Excellent communication, presentation, collaboration, and organizational skills to work as part of a team. Proven ability to think critically, adapt to new data, multi-task, and work in a fast-paced environment
Position Overview Nektar has an exciting opportunity for a Sr. Research Associate to join their team. Seek a highly motivated Senior Research Associate with a focus in Immunology for our In vitro pharmacology research team, in our San Francisco office. The individual will participate in design and conduct of experiments in biological systems to characterize and validate novel targets, to test efficacy of potential therapeutics, and to develop relevant biomarker strategies to prioritize them for further development in a variety of disease areas, including autoimmune diseases and immuno-oncology. Knowledge of flow cytometry and mammalian cell culture is required. The candidate should also possess strong knowledge of and be proficient in relevant experimental biology techniques such as immunoassays (ELISA, MSD), qPCR, biochemical/cell-based assays, and must have experience handling primary human cells. The candidate will participate in technology development in the area of in vitro assays, cell biology, and ex vivo sample testing. The individual will be expected to document experimental findings according to approved Nektar policy, and to draft associated technical reports. The individual must maintain knowledge of state-of-the-art principles and theories, develop and analyze new product concepts by reviewing preclinical and clinical literature, and participate & contribute on discovery teams, as necessary. This position contributes to and supports the company's research and development efforts to create high value therapeutics to address unmet medical needs. Essential/Primary Duties, Functions and Responsibilities Works on problems of moderate scope in which analysis of situation or data requires a review of identifiable factors. Exercises judgment within defined procedures and practices to determine appropriate action. Normally receives general instructions on routine work, detailed instructions on new assignments. Interfaces with various departments, customers and partners. Uses professional concepts and scientific knowledge to contribute to the development of company concepts and principles to achieve objectives in creative and effective ways. Defines problems, develops approaches and develops experiments with sound judgment and some supervision. Puts work practices in context with larger team. Writes reports and protocols. May act as a principal investigator in conducting own experiments. May participate in the development of patent applications. May provide guidance to junior scientific staff, providing training as needed, and ensures adherence to company policies and quality requirements. Requirements A minimum of a Bachelors' degree in a scientific discipline is required. A minimum of 8 years work experience in a research and/or development environment, preferably in industry, is required. Equivalent education and/or experience may be accepted. Previous supervisory experience is a plus. Must have excellent analytical skills, with significant hands on laboratory experience. Must have a demonstrated working knowledge of scientific principles. Must be able to demonstrate good decision-making skills. Must possess excellent oral and written communication skills. Must be able to work well in and promote a team environment, and demonstrate leadership abilities. Must be able to provide technical assistance to others in a wide range of laboratory based activities/techniques. Must be able to independently write reports. Must be able to demonstrate sound judgment within broadly defined practices and policies. M Must have demonstrated problem solving abilities. Strong organizational skills are required. Good computer skills, with working knowledge of MS word, Excel, PowerPoint and Graphpad Prism, are required. Performance of tasks essential to the job function may require the employee to work directly with, handle, or otherwise come into contact with chemicals or pharmaceutical agents. Employees may be required to enroll in a medical surveillance program based on the potential exposure to certain health hazards encountered as a result of their job function. We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. Nektar Therapeutics will consider for employment qualified applicants with criminal histories in the manner proscribed by the San Francisco Fair Chance Ordinance.
03/04/2021
Full time
Position Overview Nektar has an exciting opportunity for a Sr. Research Associate to join their team. Seek a highly motivated Senior Research Associate with a focus in Immunology for our In vitro pharmacology research team, in our San Francisco office. The individual will participate in design and conduct of experiments in biological systems to characterize and validate novel targets, to test efficacy of potential therapeutics, and to develop relevant biomarker strategies to prioritize them for further development in a variety of disease areas, including autoimmune diseases and immuno-oncology. Knowledge of flow cytometry and mammalian cell culture is required. The candidate should also possess strong knowledge of and be proficient in relevant experimental biology techniques such as immunoassays (ELISA, MSD), qPCR, biochemical/cell-based assays, and must have experience handling primary human cells. The candidate will participate in technology development in the area of in vitro assays, cell biology, and ex vivo sample testing. The individual will be expected to document experimental findings according to approved Nektar policy, and to draft associated technical reports. The individual must maintain knowledge of state-of-the-art principles and theories, develop and analyze new product concepts by reviewing preclinical and clinical literature, and participate & contribute on discovery teams, as necessary. This position contributes to and supports the company's research and development efforts to create high value therapeutics to address unmet medical needs. Essential/Primary Duties, Functions and Responsibilities Works on problems of moderate scope in which analysis of situation or data requires a review of identifiable factors. Exercises judgment within defined procedures and practices to determine appropriate action. Normally receives general instructions on routine work, detailed instructions on new assignments. Interfaces with various departments, customers and partners. Uses professional concepts and scientific knowledge to contribute to the development of company concepts and principles to achieve objectives in creative and effective ways. Defines problems, develops approaches and develops experiments with sound judgment and some supervision. Puts work practices in context with larger team. Writes reports and protocols. May act as a principal investigator in conducting own experiments. May participate in the development of patent applications. May provide guidance to junior scientific staff, providing training as needed, and ensures adherence to company policies and quality requirements. Requirements A minimum of a Bachelors' degree in a scientific discipline is required. A minimum of 8 years work experience in a research and/or development environment, preferably in industry, is required. Equivalent education and/or experience may be accepted. Previous supervisory experience is a plus. Must have excellent analytical skills, with significant hands on laboratory experience. Must have a demonstrated working knowledge of scientific principles. Must be able to demonstrate good decision-making skills. Must possess excellent oral and written communication skills. Must be able to work well in and promote a team environment, and demonstrate leadership abilities. Must be able to provide technical assistance to others in a wide range of laboratory based activities/techniques. Must be able to independently write reports. Must be able to demonstrate sound judgment within broadly defined practices and policies. M Must have demonstrated problem solving abilities. Strong organizational skills are required. Good computer skills, with working knowledge of MS word, Excel, PowerPoint and Graphpad Prism, are required. Performance of tasks essential to the job function may require the employee to work directly with, handle, or otherwise come into contact with chemicals or pharmaceutical agents. Employees may be required to enroll in a medical surveillance program based on the potential exposure to certain health hazards encountered as a result of their job function. We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. Nektar Therapeutics will consider for employment qualified applicants with criminal histories in the manner proscribed by the San Francisco Fair Chance Ordinance.
We are seeking a highly motivated and driven Research Associate who will be key in advancing our preclinical drug development and scientific initiatives. Alkahest's therapeutic focus is on indications impacted by aging, including cognition and disorders of the nervous system. The candidate will help establish in vitro cellular assays to enable bioactive screening and mechanism of action studies centered on the CNS. The candidate will be assisting Alkahest's scientific team to establish, validate, and implement scientific methodologies to understand the biology of our therapeutics at the core of the company's mission. Studies performed will involve cutting edge science and require innovative concepts and strategies. This role requires dedication to drive science, ability to multitask, excellent communication and organizational skills, attention to detail, and the ability to work independently as well as collaboratively with a multidisciplinary team of scientists and research associates to drive multiple projects in parallel in a fast-paced environment. Alkahest offers a competitive compensation and benefits package, opportunities for career growth and development, and a work environment that is collaborative, collegial, and fun. RESPONSIBLITIES: Specific responsibilities include, but are not limited to: Isolating, purifying and maintaining primary neurons, glia and neuronal progenitor cells from mouse Designing, planning, and performing cellular experiments Use of molecular and biochemical analysis methods, immunocytochemistry and microscopy Apply high throughput imaging and analysis techniques Generation and maintenance of stable cell lines Investigating and developing new methods and technologies for project advancement Data analysis and regular presentation of scientific data to the team Maintaining familiarity with current scientific literature Interfacing on a regular basis with lead scientific staff to organize, coordinate, schedule and execute experimental work. Other responsibilities as assigned REQUIREMENTS: Bachelor or Masters degree in biology, cell biology, or neuroscience is required Minimum of five (5) years of progressively advancing responsibilities and technical experience in the lab Experience with immunocytochemistry and microscopy is required Multiple years of experience with primary neuronal tissue culture and cell-based assay development is required Ability to handle and dissect mice to initiate primary cell culture is a must. Experience with other peripheral primary cells, iPSCs and stem cells/progenitor cells or endothelial cell culture systems is a plus Experience with Western Blot, FACS, qPCR and Elisa is a plus Experience with cell-based medium-throughput screening assays is a plus Excellent interpersonal, verbal and written communication skills that enable the individual to interact with all levels of management, technical and scientific personnel Attention to detail and strong organizational skills with the drive for excellence in a high paced environment Solid knowledge of Microsoft Office Suite, microscopy software and analytical packages such as Prism
01/31/2021
Full time
We are seeking a highly motivated and driven Research Associate who will be key in advancing our preclinical drug development and scientific initiatives. Alkahest's therapeutic focus is on indications impacted by aging, including cognition and disorders of the nervous system. The candidate will help establish in vitro cellular assays to enable bioactive screening and mechanism of action studies centered on the CNS. The candidate will be assisting Alkahest's scientific team to establish, validate, and implement scientific methodologies to understand the biology of our therapeutics at the core of the company's mission. Studies performed will involve cutting edge science and require innovative concepts and strategies. This role requires dedication to drive science, ability to multitask, excellent communication and organizational skills, attention to detail, and the ability to work independently as well as collaboratively with a multidisciplinary team of scientists and research associates to drive multiple projects in parallel in a fast-paced environment. Alkahest offers a competitive compensation and benefits package, opportunities for career growth and development, and a work environment that is collaborative, collegial, and fun. RESPONSIBLITIES: Specific responsibilities include, but are not limited to: Isolating, purifying and maintaining primary neurons, glia and neuronal progenitor cells from mouse Designing, planning, and performing cellular experiments Use of molecular and biochemical analysis methods, immunocytochemistry and microscopy Apply high throughput imaging and analysis techniques Generation and maintenance of stable cell lines Investigating and developing new methods and technologies for project advancement Data analysis and regular presentation of scientific data to the team Maintaining familiarity with current scientific literature Interfacing on a regular basis with lead scientific staff to organize, coordinate, schedule and execute experimental work. Other responsibilities as assigned REQUIREMENTS: Bachelor or Masters degree in biology, cell biology, or neuroscience is required Minimum of five (5) years of progressively advancing responsibilities and technical experience in the lab Experience with immunocytochemistry and microscopy is required Multiple years of experience with primary neuronal tissue culture and cell-based assay development is required Ability to handle and dissect mice to initiate primary cell culture is a must. Experience with other peripheral primary cells, iPSCs and stem cells/progenitor cells or endothelial cell culture systems is a plus Experience with Western Blot, FACS, qPCR and Elisa is a plus Experience with cell-based medium-throughput screening assays is a plus Excellent interpersonal, verbal and written communication skills that enable the individual to interact with all levels of management, technical and scientific personnel Attention to detail and strong organizational skills with the drive for excellence in a high paced environment Solid knowledge of Microsoft Office Suite, microscopy software and analytical packages such as Prism
Tentarix Biotherapeutics, Inc
San Diego, California
Tentarix Biotherapeutics is an exciting company with a game-changing technology platform that allows for the discovery of next generation, multi-specific investigational drugs to address the key limitations of existing cancer, autoimmune and other disease therapies. We are led by a seasoned executive team with proven expertise in the field of multi-specific drug discovery. We are committed to hiring and retaining highly qualified and motivated team members who are passionate about making a difference for patients in the modern drug discovery landscape with our ground-breaking technology. Tentarix team members are creative, hands-on, self-directed, and display excellent communication skills. They also exhibit a high level of integrity, resiliency and a determination to contribute to the success of the company. We offer autonomy, flexibility, a modern culture and the best tools to ensure the success of our individuals and collectively. Role: The Senior Research Associate will be primarily responsible for developing and maintaining Tentarix's mammalian protein expression platform. Additionally, this person will support molecular biology, plasmid design, construction, and characterization. Responsibilities: Primarily responsible for Tentarix's mammalian expression platform. Includes evaluating vendor expression platforms, performing transfections, and developing/optimizing multi-well, spin tube, and flask-based HEK293/CHO cultures. Secondarily, will support molecular biology design and construction of complex biologics, monoclonal and multispecific antibodies, and soluble and membrane bound receptors. Independently plan and execute experiments, analyze results, and present data at team meetings Maintain a meticulous record of their research Qualifications and Skills: B.S., plus 6 or more years or M.S plus 4 or more years of experience with recombinant protein expression using mammalian systems, ideally in an industrial setting. Prior experience with mammalian cell culture, transfection, harvest, and protein quantification. Experience with mammalian stable cell line selection for recombinant protein expression and characterization is desired. Technical experience in the development and execution of protein quantification and detection assays, such as ELISAs, is desired. Goal oriented, highly organized, and capable of multitasking Strong communication and record keeping abilities Thrive in a cross functional team environment Our employees are the Company's most valuable asset, so we offer attractive compensation and benefits packages to retain the best in the field. Tentarix is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Tentarix Biotherapeutics, Inc. also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. We are an E-Verify employer.
01/28/2021
Full time
Tentarix Biotherapeutics is an exciting company with a game-changing technology platform that allows for the discovery of next generation, multi-specific investigational drugs to address the key limitations of existing cancer, autoimmune and other disease therapies. We are led by a seasoned executive team with proven expertise in the field of multi-specific drug discovery. We are committed to hiring and retaining highly qualified and motivated team members who are passionate about making a difference for patients in the modern drug discovery landscape with our ground-breaking technology. Tentarix team members are creative, hands-on, self-directed, and display excellent communication skills. They also exhibit a high level of integrity, resiliency and a determination to contribute to the success of the company. We offer autonomy, flexibility, a modern culture and the best tools to ensure the success of our individuals and collectively. Role: The Senior Research Associate will be primarily responsible for developing and maintaining Tentarix's mammalian protein expression platform. Additionally, this person will support molecular biology, plasmid design, construction, and characterization. Responsibilities: Primarily responsible for Tentarix's mammalian expression platform. Includes evaluating vendor expression platforms, performing transfections, and developing/optimizing multi-well, spin tube, and flask-based HEK293/CHO cultures. Secondarily, will support molecular biology design and construction of complex biologics, monoclonal and multispecific antibodies, and soluble and membrane bound receptors. Independently plan and execute experiments, analyze results, and present data at team meetings Maintain a meticulous record of their research Qualifications and Skills: B.S., plus 6 or more years or M.S plus 4 or more years of experience with recombinant protein expression using mammalian systems, ideally in an industrial setting. Prior experience with mammalian cell culture, transfection, harvest, and protein quantification. Experience with mammalian stable cell line selection for recombinant protein expression and characterization is desired. Technical experience in the development and execution of protein quantification and detection assays, such as ELISAs, is desired. Goal oriented, highly organized, and capable of multitasking Strong communication and record keeping abilities Thrive in a cross functional team environment Our employees are the Company's most valuable asset, so we offer attractive compensation and benefits packages to retain the best in the field. Tentarix is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Tentarix Biotherapeutics, Inc. also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. We are an E-Verify employer.
Mammoth Biosciences is harnessing the diversity of nature to power the next-generation of CRISPR products. Through the discovery and development of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. By leveraging its internal research and development and exclusive licensing to patents related to Cas12, Cas13, Cas14 and CasPhi, Mammoth Biosciences can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Mammoth is democratizing disease detection with easy and affordable point-of-care tests that allow real-time and simultaneous detection of multiple conditions, along with high-throughput tests that allow for unprecedented testing volume. Further, the company is transforming disease treatment with its proprietary micro-sized CRISPR proteins that enable new editing and delivery options. Based in San Francisco, Mammoth Biosciences is co-founded by CRISPR pioneer Jennifer Doudna and principal founders Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Decheng, Mayfield, NFX, and 8VC, and leading individual investors including Brook Byers, Tim Cook, Bob Nelsen, and Jeff Huber. At Mammoth, we are passionate about transforming lives through innovation. DESCRIPTION Mammoth is currently seeking a talented and detail-oriented Research Associate with experience in molecular biology, flow cytometry, mammalian cell culture, and genetic manipulation of immune cells and/or iPSCs, to join our growing genome editing team. The successful candidate will work closely and collaboratively with colleagues to design/develop, perform and execute experiments on mammalian cell lines and primary human cells to support pipeline projects to develop Cas nucleases for genome editing. They will have the opportunity to contribute broadly across multiple technical areas. The position requires adaptability, excellent planning, strong organizational and communication skills, as well as the ability to work independently in a fast-paced team-oriented and dynamic environment. Responsibilities Execution of molecular biology, cloning, cell culture and a broad range of functional and phenotypic assay workflows to accelerate projects in pre-clinical development. Provide team support for basic laboratory tasks including tissue culture of various cell lines, isolation and culture of primary human cells from whole blood, phenotyping and analytical characterization of cells, genome editing of primary human cells, and virus production Accurately and timely record, document, analyze and communicate laboratory data to senior staff and team members Conducts all activities in a safe and efficient manner, and adheres to site environmental health and safety (EHS) requirements Requirements BS or MS or in molecular and cell biology, Immunology, biochemistry, or a related field, plus at least 3 years relevant laboratory research experience. Industry work experience, especially in immunology/immuno-oncology field, is highly desirable. Proficiency in mammalian cell culture, isolation and culture and genetic manipulation of primary human immune cells.Previous work experience with human NK and T cells and/or CAR-T cell production and characterization is highly desirable. Proficiency with multi-color flow cytometry, FACS sorting and T-cell based assays (T cell phenotyping and memory sub-setting, cytotoxicity, cell proliferation, cytokine secretion). Proficiency in molecular cloning, PCR, qPCR, genomic DNA and mRNA purification, NGS sequencing, ELISA, western blot, retroviral/lentiviral/AAV production, titration, and transduction. Strong organizational and record-keeping skills, excellent interpersonal, verbal and written communication skills. Highly motivated with strong work ethics. Ability to work efficiently and productively in a fast-paced highly collaborative team environment Knowledge of Microsoft Office Suite and other relevant data analysis software (Flowjo, GraphPad Prism, etc.). Benefits Competitive salary Full health/vision/dental benefits Unlimited vacation Free breakfast, lunch, dinner, and barista Free gym Public transportation commuter subsidy 401(k) matching
01/28/2021
Full time
Mammoth Biosciences is harnessing the diversity of nature to power the next-generation of CRISPR products. Through the discovery and development of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. By leveraging its internal research and development and exclusive licensing to patents related to Cas12, Cas13, Cas14 and CasPhi, Mammoth Biosciences can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Mammoth is democratizing disease detection with easy and affordable point-of-care tests that allow real-time and simultaneous detection of multiple conditions, along with high-throughput tests that allow for unprecedented testing volume. Further, the company is transforming disease treatment with its proprietary micro-sized CRISPR proteins that enable new editing and delivery options. Based in San Francisco, Mammoth Biosciences is co-founded by CRISPR pioneer Jennifer Doudna and principal founders Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Decheng, Mayfield, NFX, and 8VC, and leading individual investors including Brook Byers, Tim Cook, Bob Nelsen, and Jeff Huber. At Mammoth, we are passionate about transforming lives through innovation. DESCRIPTION Mammoth is currently seeking a talented and detail-oriented Research Associate with experience in molecular biology, flow cytometry, mammalian cell culture, and genetic manipulation of immune cells and/or iPSCs, to join our growing genome editing team. The successful candidate will work closely and collaboratively with colleagues to design/develop, perform and execute experiments on mammalian cell lines and primary human cells to support pipeline projects to develop Cas nucleases for genome editing. They will have the opportunity to contribute broadly across multiple technical areas. The position requires adaptability, excellent planning, strong organizational and communication skills, as well as the ability to work independently in a fast-paced team-oriented and dynamic environment. Responsibilities Execution of molecular biology, cloning, cell culture and a broad range of functional and phenotypic assay workflows to accelerate projects in pre-clinical development. Provide team support for basic laboratory tasks including tissue culture of various cell lines, isolation and culture of primary human cells from whole blood, phenotyping and analytical characterization of cells, genome editing of primary human cells, and virus production Accurately and timely record, document, analyze and communicate laboratory data to senior staff and team members Conducts all activities in a safe and efficient manner, and adheres to site environmental health and safety (EHS) requirements Requirements BS or MS or in molecular and cell biology, Immunology, biochemistry, or a related field, plus at least 3 years relevant laboratory research experience. Industry work experience, especially in immunology/immuno-oncology field, is highly desirable. Proficiency in mammalian cell culture, isolation and culture and genetic manipulation of primary human immune cells.Previous work experience with human NK and T cells and/or CAR-T cell production and characterization is highly desirable. Proficiency with multi-color flow cytometry, FACS sorting and T-cell based assays (T cell phenotyping and memory sub-setting, cytotoxicity, cell proliferation, cytokine secretion). Proficiency in molecular cloning, PCR, qPCR, genomic DNA and mRNA purification, NGS sequencing, ELISA, western blot, retroviral/lentiviral/AAV production, titration, and transduction. Strong organizational and record-keeping skills, excellent interpersonal, verbal and written communication skills. Highly motivated with strong work ethics. Ability to work efficiently and productively in a fast-paced highly collaborative team environment Knowledge of Microsoft Office Suite and other relevant data analysis software (Flowjo, GraphPad Prism, etc.). Benefits Competitive salary Full health/vision/dental benefits Unlimited vacation Free breakfast, lunch, dinner, and barista Free gym Public transportation commuter subsidy 401(k) matching
Vaxcyte, Inc. (Nasdaq: PCVX) is a next-generation vaccine company seeking to improve global health by developing superior and novel vaccines designed to prevent some of the most common and deadly infectious diseases worldwide. Our exclusively licensed cell-free protein synthesis platform and our proprietary know how enable us to design and produce optimized protein carriers and antigens, the critical building blocks of vaccines, in ways that we believe conventional vaccine technologies cannot. Our pipeline includes pneumococcal conjugate vaccine, or PCV, candidates that we believe are the most broad- spectrum PCV candidates currently in development, targeting the $7 billion global pneumococcal vaccine market. Our lead vaccine candidate, VAX-24, is a preclinical, 24-valent broad-spectrum pneumococcal conjugate PCV with preclinical proof-of-concept demonstrating potential to replace the standard of care that we expect to advance into clinical trials in the second half of 2021. Our pipeline also includes VAX- XP, a PCV with an expanded breadth of coverage of at least 30 strains, including newly emerging strains responsible for invasive pneumococcal disease and antibiotic resistance; VAX-A1, a prophylactic vaccine candidate designed to prevent Group A Strep infections; and VAX-PG, a therapeutic vaccine candidate designed to slow or stop the progression of periodontal disease by targeting the keystone pathogen responsible for this chronic, oral inflammatory disease. We completed our initial public offering in June 2020, raising $287.5 million in gross proceeds. Summary: Vaxcyte is looking for an energetic and talented individual to join our Formulation and Drug Product Development team. Candidate should be an experienced and flexible individual who would be comfortable working on multiple tasks at once, with excellent communication skills to navigate rapid changes in prioritization. This position is for a scientific role which will be primarily lab based. The candidate will have a good knowledge and practical experience in the field of vaccine formulation. The candidate will also have industry based experience in a range of standard protein biochemistry analytical techniques. Experience in biophysical characterization of formulations would be very beneficial. The primary responsibility for the incumbent will be to support development of projects from the research through the preclinical phase, into early clinical phases and also supporting later stage programs. Essential Functions: • Supporting formulation manufacture for thermal, freeze/thaw, and agitation stability studies on both Drug Substance (DS) and Drug Product (DP) materials • Conducting analysis of these formulations using a broad range of standard protein biochemistry analytical techniques for formulation selection • Supporting formulation comparability studies, container selection and scale up activities • Preparing sterile formulation samples to be used for in vivo testing • Evaluating effect of new formulation components on already established standard analytical assays • Maintaining an accurate and up to date inventory of supplies of DS used by multiple groups Requirements: • BSc or MSc in Pharmaceutics, Chemistry, Biophysics or Biochemistry preferred, or Analytical Chemistry or Biology considered, with >2years of industrial experience • The candidate will have had previous experience in developing formulation of proteins, polysaccharides and/or protein conjugates for adjuvant containing vaccines (other biological experience also considered) • Experience supporting stability studies, including study set up and sample testing and analysis • Experience in using standard protein plate based assays is needed. Some experience in spectrometric methodologies, particle light scattering, quantitative subvisible particle analysis, visible analysis and HPLC/UV is preferred • Experience working under aseptic conditions is necessary • Strong interpersonal skills; ability to communicate effectively both verbally and in written formats (must be able to work with managerial and lab team in remote setting when needed) are necessary to collaborate effectively with the rest of the Formulation and Drug Product Development team • Critical thinking, the ability to analyse data and to report results to the team in a clear and concise manner during group meetings is needed • Eagerness, ability to learn and great flexibility to rapidly switch gears between tasks • Detail oriented, rigorous and excellent skills in record keeping/documentation • Good proficiency in the use of Microsoft office suite (in particular Excel and PowerPoint) Reports to: Senior Scientist in the Formulation and Drug Product Development team Location: Foster City, CA Compensation: The compensation package will be competitive and includes comprehensive benefits and an equity component.
01/27/2021
Full time
Vaxcyte, Inc. (Nasdaq: PCVX) is a next-generation vaccine company seeking to improve global health by developing superior and novel vaccines designed to prevent some of the most common and deadly infectious diseases worldwide. Our exclusively licensed cell-free protein synthesis platform and our proprietary know how enable us to design and produce optimized protein carriers and antigens, the critical building blocks of vaccines, in ways that we believe conventional vaccine technologies cannot. Our pipeline includes pneumococcal conjugate vaccine, or PCV, candidates that we believe are the most broad- spectrum PCV candidates currently in development, targeting the $7 billion global pneumococcal vaccine market. Our lead vaccine candidate, VAX-24, is a preclinical, 24-valent broad-spectrum pneumococcal conjugate PCV with preclinical proof-of-concept demonstrating potential to replace the standard of care that we expect to advance into clinical trials in the second half of 2021. Our pipeline also includes VAX- XP, a PCV with an expanded breadth of coverage of at least 30 strains, including newly emerging strains responsible for invasive pneumococcal disease and antibiotic resistance; VAX-A1, a prophylactic vaccine candidate designed to prevent Group A Strep infections; and VAX-PG, a therapeutic vaccine candidate designed to slow or stop the progression of periodontal disease by targeting the keystone pathogen responsible for this chronic, oral inflammatory disease. We completed our initial public offering in June 2020, raising $287.5 million in gross proceeds. Summary: Vaxcyte is looking for an energetic and talented individual to join our Formulation and Drug Product Development team. Candidate should be an experienced and flexible individual who would be comfortable working on multiple tasks at once, with excellent communication skills to navigate rapid changes in prioritization. This position is for a scientific role which will be primarily lab based. The candidate will have a good knowledge and practical experience in the field of vaccine formulation. The candidate will also have industry based experience in a range of standard protein biochemistry analytical techniques. Experience in biophysical characterization of formulations would be very beneficial. The primary responsibility for the incumbent will be to support development of projects from the research through the preclinical phase, into early clinical phases and also supporting later stage programs. Essential Functions: • Supporting formulation manufacture for thermal, freeze/thaw, and agitation stability studies on both Drug Substance (DS) and Drug Product (DP) materials • Conducting analysis of these formulations using a broad range of standard protein biochemistry analytical techniques for formulation selection • Supporting formulation comparability studies, container selection and scale up activities • Preparing sterile formulation samples to be used for in vivo testing • Evaluating effect of new formulation components on already established standard analytical assays • Maintaining an accurate and up to date inventory of supplies of DS used by multiple groups Requirements: • BSc or MSc in Pharmaceutics, Chemistry, Biophysics or Biochemistry preferred, or Analytical Chemistry or Biology considered, with >2years of industrial experience • The candidate will have had previous experience in developing formulation of proteins, polysaccharides and/or protein conjugates for adjuvant containing vaccines (other biological experience also considered) • Experience supporting stability studies, including study set up and sample testing and analysis • Experience in using standard protein plate based assays is needed. Some experience in spectrometric methodologies, particle light scattering, quantitative subvisible particle analysis, visible analysis and HPLC/UV is preferred • Experience working under aseptic conditions is necessary • Strong interpersonal skills; ability to communicate effectively both verbally and in written formats (must be able to work with managerial and lab team in remote setting when needed) are necessary to collaborate effectively with the rest of the Formulation and Drug Product Development team • Critical thinking, the ability to analyse data and to report results to the team in a clear and concise manner during group meetings is needed • Eagerness, ability to learn and great flexibility to rapidly switch gears between tasks • Detail oriented, rigorous and excellent skills in record keeping/documentation • Good proficiency in the use of Microsoft office suite (in particular Excel and PowerPoint) Reports to: Senior Scientist in the Formulation and Drug Product Development team Location: Foster City, CA Compensation: The compensation package will be competitive and includes comprehensive benefits and an equity component.
At Synthetic Genomics, Inc., we are dedicated to developing and commercializing genomic-driven solutions to address global challenges. We are currently seeking a Temporary Research Associate in Biochemistry and Analytical Chemistry to join our dynamic and collaborative team in La Jolla, CA. The Temporary Research Associate will report to a senior scientist and focus on the research and development of novel host production systems for biotechnological applications. The successful candidate will perform biochemical and analytical chemistry work to analyze and evaluate engineered strains of interest. The successful candidate will have practical knowledge of protein biochemistry. Essential Duties & Responsibilities Analytic evaluation of Prokaryotic and Eukaryotic strains in collaboration with other project members. Small scale cultivation of strains including media preparation and culture maintenance. Practical working knowledge of protein biochemistry (protein extraction and analysis, LCMS, GCMS, etc ). Generate detailed and complete notebook and communicate data to group in written and oral reports. Interact effectively within a multidisciplinary team. Requirements Requires a bachelors or Master's degree in Biochemistry, Molecular Biology, Microbiology or a related field with a minimum of 2 years of experience in industry or an academic lab. Basic biochemical and analytical techniques (Protein extraction, SDS-page, Western Blotting, chromatography (LC, GC), enzymatic activity assays, etc ). Proven ability to budget time and manage several distinct projects at once. Eagerness to develop, learn and adopt new methods. Experience working with analytical instrumentation is preferred.
01/26/2021
Full time
At Synthetic Genomics, Inc., we are dedicated to developing and commercializing genomic-driven solutions to address global challenges. We are currently seeking a Temporary Research Associate in Biochemistry and Analytical Chemistry to join our dynamic and collaborative team in La Jolla, CA. The Temporary Research Associate will report to a senior scientist and focus on the research and development of novel host production systems for biotechnological applications. The successful candidate will perform biochemical and analytical chemistry work to analyze and evaluate engineered strains of interest. The successful candidate will have practical knowledge of protein biochemistry. Essential Duties & Responsibilities Analytic evaluation of Prokaryotic and Eukaryotic strains in collaboration with other project members. Small scale cultivation of strains including media preparation and culture maintenance. Practical working knowledge of protein biochemistry (protein extraction and analysis, LCMS, GCMS, etc ). Generate detailed and complete notebook and communicate data to group in written and oral reports. Interact effectively within a multidisciplinary team. Requirements Requires a bachelors or Master's degree in Biochemistry, Molecular Biology, Microbiology or a related field with a minimum of 2 years of experience in industry or an academic lab. Basic biochemical and analytical techniques (Protein extraction, SDS-page, Western Blotting, chromatography (LC, GC), enzymatic activity assays, etc ). Proven ability to budget time and manage several distinct projects at once. Eagerness to develop, learn and adopt new methods. Experience working with analytical instrumentation is preferred.
Vaxcyte, Inc. (Nasdaq: PCVX) is a next-generation vaccine company seeking to improve global health by developing superior and novel vaccines designed to prevent some of the most common and deadly infectious diseases worldwide. Our exclusively licensed cell-free protein synthesis platform and our proprietary know how enable us to design and produce optimized protein carriers and antigens, the critical building blocks of vaccines, in ways that we believe conventional vaccine technologies cannot. Our pipeline includes pneumococcal conjugate vaccine, or PCV, candidate s that we believe are the most broad-spectrum PCV candidates currently in development, targeting the $7 billion global pneumococcal vaccine market. Our lead vaccine candidate, VAX-24, is a preclinical, 24-valent broad-spectrum pneumococcal conjugate PCV with preclinical proof-of- concept demonstrating potential to replace the standard of care that we expect to advance into clinical trials in the second half of 2021. Our pipeline also includes VAX-XP, a PCV with an expanded breadth of coverage of at least 30 strains, including newly emerging strains responsible for invasive pneumococcal disease and antibiotic resistance; VAX-A1, a prophylactic vaccine candidate designed to prevent Group A Strep infections; and VAX-PG, a therapeutic vaccine candidate designed to slow or stop the progression of periodontal disease by targeting the keystone pathogen responsible for this chronic, oral inflammatory disease. We completed our initial public offering in June 2020, raising $287.5 million in gross proceeds. Summary: Vaxcyte is looking for an energetic, highly skilled and talented individual to join Vaxcyte's Vaccine Product Development organization as an Associate Director/Director within Analytical Development Quality Control department. The primary function of this Director level position in to lead the development and optimization of bioassays for the lot release, characterization, and stability and formulation assessment of conjugate vaccine intermediates, and drug substance and drug product in preclinical and clinical development. The successful candidate will also manage and build a growing group of scientists and research associates and oversee work performed in CROs. Essential Functions: • Take on end- to-end strategic responsibility for bioassay method development of intermediates, drug substance and drug products. • Lead a team of 4-5 scientists and research associate to develop, optimize, and qualify phase-appropriate a broad spectrum of immunoassay for release, stability, and formulation optimization • Drive analytical innovation and stay on top of cutting edge bioassay technology, apply innovative approaches to problem solving, and introduce and establish novel technology in house if necessary. • Collaborate with CROs to develop antibody critical reagents for the immunoassay development • Provide technical tactics and oversight of antibody purification to optimize the immunoassay performance • Develop and implement critical reagent qualification plans • Manage scientists of various levels, provide technical direction, mentorship and coaching • Cultivate a cohesive, innovative, nimble and productive team environment • Support Associate Director of QC for GMP analytical method transfer and validation at the CMOs, as well as trouble shooting of QC testing or method related issue • Collaborate with Formulation and Conjugation team to design and develop phase appropriate bioassay for in-process control and GMP quality control purpose. • Author SOPs, technical reports and multiple regulatory submission when needed, and address health authority questions during various clinical phase filings • Serves as a scientific liaison for outsourced assay development, technology transfer and data management in a CRO or CMO/CDMO environment • Evaluate and establish contracts with CDMO/CROs for method development and GMP testing or stability studies • Manage relationships with CDMOs, including managing timelines and cost for the analytical method and GMP testing. • Provide appropriate CRO/CDMO oversight by reviewing analytical method development data, reviewing and approving analytical method development report, method validation protocols, reports, and analytical method SOPs Requirements: • PhD in Biochemistry, Analytical Chemistry or Cell Biology, with 10+ years relevant industry experience; MS or BS with 15+ years of industry experience in Pharma/Diagnostic industry required • Must be a team player, strong critical thinker, exhibit a willingness to meet project timelines and multitask effectively in a dynamic fast-paced environment under challenging timelines • Proven track record of heading bioassay development for biologics, leading teams while managing multiple projects, experience building team and lab is highly desirable • A strong scientific leader who can independently design, execute, and guide effective experiments with clear goals of developing robust germane bioassay methods. and effectively and skilfully troubleshoot bioassay issue during method development, transfer, validation and QC testing in a fast pace environment • A proven track record of solving complex immunoassay and bioanalytical problems • Experiences in hybridoma technology including optimizing the immunization and fusion process, assay development, lead antibody selection and characterization. • Efficient in Interpreting data and communicating project status or risk mitigation strategies that drive critical decisions in CMC cross functional team • Demonstrated leadership and track record of successfully developing different format of bioassays by utilizing appropriate methodologies • Thorough understanding and hands on experience of MSD platform is highly desirable • Fundamental understanding of key immunology concepts related to assessment of vaccine- induced responses required • Solid understanding of relevant FDA, EU, and ICH regulatory guidelines an d pharmacopeia as applicable to immunoassay method qualification/validation for biologics and vaccines, and demonstrated ability of applying the regulatory guidance to formulate practical solutions and phase appropriate analytical strategy • Experience in IND, NDA and BLA submission is preferred • Project management skills including the ability to manage project resource requirements (material, manpower, time, etc.), and ability to elevate relevant issues to project lead and line-management • Fundamental understanding of statistics and data analysis software, proficient with analytical software such as Softmax Pro and familiar with statistical software such as JMP • Strong interpersonal skills; ability to communicate effectively both verbally and in written formats • Strong attention to detail supported by excellent time management and organizational skills Reports to: Senior Director, Analytical Development & Quality Control Location: Foster City, CA Compensation: The compensation package will be competitive and includes comprehensive benefits and an equity component.
01/26/2021
Full time
Vaxcyte, Inc. (Nasdaq: PCVX) is a next-generation vaccine company seeking to improve global health by developing superior and novel vaccines designed to prevent some of the most common and deadly infectious diseases worldwide. Our exclusively licensed cell-free protein synthesis platform and our proprietary know how enable us to design and produce optimized protein carriers and antigens, the critical building blocks of vaccines, in ways that we believe conventional vaccine technologies cannot. Our pipeline includes pneumococcal conjugate vaccine, or PCV, candidate s that we believe are the most broad-spectrum PCV candidates currently in development, targeting the $7 billion global pneumococcal vaccine market. Our lead vaccine candidate, VAX-24, is a preclinical, 24-valent broad-spectrum pneumococcal conjugate PCV with preclinical proof-of- concept demonstrating potential to replace the standard of care that we expect to advance into clinical trials in the second half of 2021. Our pipeline also includes VAX-XP, a PCV with an expanded breadth of coverage of at least 30 strains, including newly emerging strains responsible for invasive pneumococcal disease and antibiotic resistance; VAX-A1, a prophylactic vaccine candidate designed to prevent Group A Strep infections; and VAX-PG, a therapeutic vaccine candidate designed to slow or stop the progression of periodontal disease by targeting the keystone pathogen responsible for this chronic, oral inflammatory disease. We completed our initial public offering in June 2020, raising $287.5 million in gross proceeds. Summary: Vaxcyte is looking for an energetic, highly skilled and talented individual to join Vaxcyte's Vaccine Product Development organization as an Associate Director/Director within Analytical Development Quality Control department. The primary function of this Director level position in to lead the development and optimization of bioassays for the lot release, characterization, and stability and formulation assessment of conjugate vaccine intermediates, and drug substance and drug product in preclinical and clinical development. The successful candidate will also manage and build a growing group of scientists and research associates and oversee work performed in CROs. Essential Functions: • Take on end- to-end strategic responsibility for bioassay method development of intermediates, drug substance and drug products. • Lead a team of 4-5 scientists and research associate to develop, optimize, and qualify phase-appropriate a broad spectrum of immunoassay for release, stability, and formulation optimization • Drive analytical innovation and stay on top of cutting edge bioassay technology, apply innovative approaches to problem solving, and introduce and establish novel technology in house if necessary. • Collaborate with CROs to develop antibody critical reagents for the immunoassay development • Provide technical tactics and oversight of antibody purification to optimize the immunoassay performance • Develop and implement critical reagent qualification plans • Manage scientists of various levels, provide technical direction, mentorship and coaching • Cultivate a cohesive, innovative, nimble and productive team environment • Support Associate Director of QC for GMP analytical method transfer and validation at the CMOs, as well as trouble shooting of QC testing or method related issue • Collaborate with Formulation and Conjugation team to design and develop phase appropriate bioassay for in-process control and GMP quality control purpose. • Author SOPs, technical reports and multiple regulatory submission when needed, and address health authority questions during various clinical phase filings • Serves as a scientific liaison for outsourced assay development, technology transfer and data management in a CRO or CMO/CDMO environment • Evaluate and establish contracts with CDMO/CROs for method development and GMP testing or stability studies • Manage relationships with CDMOs, including managing timelines and cost for the analytical method and GMP testing. • Provide appropriate CRO/CDMO oversight by reviewing analytical method development data, reviewing and approving analytical method development report, method validation protocols, reports, and analytical method SOPs Requirements: • PhD in Biochemistry, Analytical Chemistry or Cell Biology, with 10+ years relevant industry experience; MS or BS with 15+ years of industry experience in Pharma/Diagnostic industry required • Must be a team player, strong critical thinker, exhibit a willingness to meet project timelines and multitask effectively in a dynamic fast-paced environment under challenging timelines • Proven track record of heading bioassay development for biologics, leading teams while managing multiple projects, experience building team and lab is highly desirable • A strong scientific leader who can independently design, execute, and guide effective experiments with clear goals of developing robust germane bioassay methods. and effectively and skilfully troubleshoot bioassay issue during method development, transfer, validation and QC testing in a fast pace environment • A proven track record of solving complex immunoassay and bioanalytical problems • Experiences in hybridoma technology including optimizing the immunization and fusion process, assay development, lead antibody selection and characterization. • Efficient in Interpreting data and communicating project status or risk mitigation strategies that drive critical decisions in CMC cross functional team • Demonstrated leadership and track record of successfully developing different format of bioassays by utilizing appropriate methodologies • Thorough understanding and hands on experience of MSD platform is highly desirable • Fundamental understanding of key immunology concepts related to assessment of vaccine- induced responses required • Solid understanding of relevant FDA, EU, and ICH regulatory guidelines an d pharmacopeia as applicable to immunoassay method qualification/validation for biologics and vaccines, and demonstrated ability of applying the regulatory guidance to formulate practical solutions and phase appropriate analytical strategy • Experience in IND, NDA and BLA submission is preferred • Project management skills including the ability to manage project resource requirements (material, manpower, time, etc.), and ability to elevate relevant issues to project lead and line-management • Fundamental understanding of statistics and data analysis software, proficient with analytical software such as Softmax Pro and familiar with statistical software such as JMP • Strong interpersonal skills; ability to communicate effectively both verbally and in written formats • Strong attention to detail supported by excellent time management and organizational skills Reports to: Senior Director, Analytical Development & Quality Control Location: Foster City, CA Compensation: The compensation package will be competitive and includes comprehensive benefits and an equity component.
Position Overview Nektar has an exciting opportunity for a Sr. Research Associate to join their team. Seek a highly motivated Senior Research Associate with a focus in Immunology for our In vitro pharmacology research team, in our San Francisco office. The individual will participate in design and conduct of experiments in biological systems to characterize and validate novel targets, to test efficacy of potential therapeutics, and to develop relevant biomarker strategies to prioritize them for further development in a variety of disease areas, including autoimmune diseases and immuno-oncology. Knowledge of flow cytometry and mammalian cell culture is required. The candidate should also possess strong knowledge of and be proficient in relevant experimental biology techniques such as immunoassays (ELISA, MSD), qPCR, biochemical/cell-based assays, and must have experience handling primary human cells. The candidate will participate in technology development in the area of in vitro assays, cell biology, and ex vivo sample testing. The individual will be expected to document experimental findings according to approved Nektar policy, and to draft associated technical reports. The individual must maintain knowledge of state-of-the-art principles and theories, develop and analyze new product concepts by reviewing preclinical and clinical literature, and participate & contribute on discovery teams, as necessary. This position contributes to and supports the company's research and development efforts to create high value therapeutics to address unmet medical needs. Essential/Primary Duties, Functions and Responsibilities Works on problems of moderate scope in which analysis of situation or data requires a review of identifiable factors. Exercises judgment within defined procedures and practices to determine appropriate action. Normally receives general instructions on routine work, detailed instructions on new assignments. Interfaces with various departments, customers and partners. Uses professional concepts and scientific knowledge to contribute to the development of company concepts and principles to achieve objectives in creative and effective ways. Defines problems, develops approaches and develops experiments with sound judgment and some supervision. Puts work practices in context with larger team. Writes reports and protocols. May act as a principal investigator in conducting own experiments. May participate in the development of patent applications. May provide guidance to junior scientific staff, providing training as needed, and ensures adherence to company policies and quality requirements. Requirements A minimum of a Bachelors' degree in a scientific discipline is required. A minimum of 8 years work experience in a research and/or development environment, preferably in industry, is required. Equivalent education and/or experience may be accepted. Previous supervisory experience is a plus. Must have excellent analytical skills, with significant hands on laboratory experience. Must have a demonstrated working knowledge of scientific principles. Must be able to demonstrate good decision-making skills. Must possess excellent oral and written communication skills. Must be able to work well in and promote a team environment, and demonstrate leadership abilities. Must be able to provide technical assistance to others in a wide range of laboratory based activities/techniques. Must be able to independently write reports. Must be able to demonstrate sound judgment within broadly defined practices and policies. M Must have demonstrated problem solving abilities. Strong organizational skills are required. Good computer skills, with working knowledge of MS word, Excel, PowerPoint and Graphpad Prism, are required. Performance of tasks essential to the job function may require the employee to work directly with, handle, or otherwise come into contact with chemicals or pharmaceutical agents. Employees may be required to enroll in a medical surveillance program based on the potential exposure to certain health hazards encountered as a result of their job function. We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. Nektar Therapeutics will consider for employment qualified applicants with criminal histories in the manner proscribed by the San Francisco Fair Chance Ordinance.
01/26/2021
Full time
Position Overview Nektar has an exciting opportunity for a Sr. Research Associate to join their team. Seek a highly motivated Senior Research Associate with a focus in Immunology for our In vitro pharmacology research team, in our San Francisco office. The individual will participate in design and conduct of experiments in biological systems to characterize and validate novel targets, to test efficacy of potential therapeutics, and to develop relevant biomarker strategies to prioritize them for further development in a variety of disease areas, including autoimmune diseases and immuno-oncology. Knowledge of flow cytometry and mammalian cell culture is required. The candidate should also possess strong knowledge of and be proficient in relevant experimental biology techniques such as immunoassays (ELISA, MSD), qPCR, biochemical/cell-based assays, and must have experience handling primary human cells. The candidate will participate in technology development in the area of in vitro assays, cell biology, and ex vivo sample testing. The individual will be expected to document experimental findings according to approved Nektar policy, and to draft associated technical reports. The individual must maintain knowledge of state-of-the-art principles and theories, develop and analyze new product concepts by reviewing preclinical and clinical literature, and participate & contribute on discovery teams, as necessary. This position contributes to and supports the company's research and development efforts to create high value therapeutics to address unmet medical needs. Essential/Primary Duties, Functions and Responsibilities Works on problems of moderate scope in which analysis of situation or data requires a review of identifiable factors. Exercises judgment within defined procedures and practices to determine appropriate action. Normally receives general instructions on routine work, detailed instructions on new assignments. Interfaces with various departments, customers and partners. Uses professional concepts and scientific knowledge to contribute to the development of company concepts and principles to achieve objectives in creative and effective ways. Defines problems, develops approaches and develops experiments with sound judgment and some supervision. Puts work practices in context with larger team. Writes reports and protocols. May act as a principal investigator in conducting own experiments. May participate in the development of patent applications. May provide guidance to junior scientific staff, providing training as needed, and ensures adherence to company policies and quality requirements. Requirements A minimum of a Bachelors' degree in a scientific discipline is required. A minimum of 8 years work experience in a research and/or development environment, preferably in industry, is required. Equivalent education and/or experience may be accepted. Previous supervisory experience is a plus. Must have excellent analytical skills, with significant hands on laboratory experience. Must have a demonstrated working knowledge of scientific principles. Must be able to demonstrate good decision-making skills. Must possess excellent oral and written communication skills. Must be able to work well in and promote a team environment, and demonstrate leadership abilities. Must be able to provide technical assistance to others in a wide range of laboratory based activities/techniques. Must be able to independently write reports. Must be able to demonstrate sound judgment within broadly defined practices and policies. M Must have demonstrated problem solving abilities. Strong organizational skills are required. Good computer skills, with working knowledge of MS word, Excel, PowerPoint and Graphpad Prism, are required. Performance of tasks essential to the job function may require the employee to work directly with, handle, or otherwise come into contact with chemicals or pharmaceutical agents. Employees may be required to enroll in a medical surveillance program based on the potential exposure to certain health hazards encountered as a result of their job function. We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. Nektar Therapeutics will consider for employment qualified applicants with criminal histories in the manner proscribed by the San Francisco Fair Chance Ordinance.
About Us: We are Larimar Therapeutics, based in the Philadelphia area, and committed to discovering and developing therapeutics to dramatically improve the lives of patients with complex rare diseases. At Larimar we embrace everyone's contribution to our Mission, have fun while working passionately. Our values connect us and ensure we communicate openly, treat each other with respect, help us grow from our successes and failures, and innovate. If you are searching for a company where urgency, agility, and commitment to science win the day - we welcome you! Position Summary: We are seeking an outstanding Senior Research Associate to work in our discovery and pipeline development group to help develop and implement gene expression/biomarker strategies to support clinical programs and preclinical studies. This role reports to the Associate Director in charge of Discovery and will be responsible for the design and execution of all activities related to drug discovery. Responsibilities: Will include but are not limited to: Performing advance development of assays for high throughput gene expression profiling and analyses using platforms such as qRT-PCR and NanoString Collaborating with preclinical and clinical researchers to develop best practice and SOPs including all aspects of samples' handling. Develop well defined schedule for clinical biomarker gene expression assays to achieve timely delivery of data while maximize laboratory efficiency Timely executing gene expression profiling studies and data analyses Providing subject matter expertise and work with cross-functional teams in discovery biology to develop gene expression biomarker strategy for preclinical studies Designing and executing NanoString assays for biomarker research to support multiple discovery programs Providing oversight of development and production of reagents from external vendors for the outsourced gene expression studies Working closely with data analysis teams and external statistical experts and bioinformaticians to Participating in discovery project activities outside of gene expression analysis areas Drafting experimental plans, validation reports, and study summaries Keeping current of electronic notebook record of experiments and procedures Education, Experience, Skills and Knowledge: This role requires a BS or MS in molecular biology, cell biology, or related life science disciplines with a demonstrated expertise in gene expression regulatory mechanisms, with a minimum of 8 (B.Sc.) or 5 (M.Sc.) years of experience in the pharmaceutical or biotechnology industry. Proven hand-on experience in RNA/protein isolation and quantification, and a minimum of 2 years of experience in designing, conducting, and analyzing NanoString assay and qRT-PCR assay independently is also required. Prior experience in clinical biomarker research in the biotech / pharmaceutical industry or academic genomics center is preferred, as is a background or experience in neuroscience. The successful candidate will have the ability to interpret and report gene expression data in a concise and accurate manner and be able to effectively communicate the needs of the company's programs to external collaborators as well as different stakeholders within the company.
01/26/2021
Full time
About Us: We are Larimar Therapeutics, based in the Philadelphia area, and committed to discovering and developing therapeutics to dramatically improve the lives of patients with complex rare diseases. At Larimar we embrace everyone's contribution to our Mission, have fun while working passionately. Our values connect us and ensure we communicate openly, treat each other with respect, help us grow from our successes and failures, and innovate. If you are searching for a company where urgency, agility, and commitment to science win the day - we welcome you! Position Summary: We are seeking an outstanding Senior Research Associate to work in our discovery and pipeline development group to help develop and implement gene expression/biomarker strategies to support clinical programs and preclinical studies. This role reports to the Associate Director in charge of Discovery and will be responsible for the design and execution of all activities related to drug discovery. Responsibilities: Will include but are not limited to: Performing advance development of assays for high throughput gene expression profiling and analyses using platforms such as qRT-PCR and NanoString Collaborating with preclinical and clinical researchers to develop best practice and SOPs including all aspects of samples' handling. Develop well defined schedule for clinical biomarker gene expression assays to achieve timely delivery of data while maximize laboratory efficiency Timely executing gene expression profiling studies and data analyses Providing subject matter expertise and work with cross-functional teams in discovery biology to develop gene expression biomarker strategy for preclinical studies Designing and executing NanoString assays for biomarker research to support multiple discovery programs Providing oversight of development and production of reagents from external vendors for the outsourced gene expression studies Working closely with data analysis teams and external statistical experts and bioinformaticians to Participating in discovery project activities outside of gene expression analysis areas Drafting experimental plans, validation reports, and study summaries Keeping current of electronic notebook record of experiments and procedures Education, Experience, Skills and Knowledge: This role requires a BS or MS in molecular biology, cell biology, or related life science disciplines with a demonstrated expertise in gene expression regulatory mechanisms, with a minimum of 8 (B.Sc.) or 5 (M.Sc.) years of experience in the pharmaceutical or biotechnology industry. Proven hand-on experience in RNA/protein isolation and quantification, and a minimum of 2 years of experience in designing, conducting, and analyzing NanoString assay and qRT-PCR assay independently is also required. Prior experience in clinical biomarker research in the biotech / pharmaceutical industry or academic genomics center is preferred, as is a background or experience in neuroscience. The successful candidate will have the ability to interpret and report gene expression data in a concise and accurate manner and be able to effectively communicate the needs of the company's programs to external collaborators as well as different stakeholders within the company.
Company Summary: Cellarity, Inc. is a privately held, well-funded, early-stage biotechnology company that is pioneering a novel transformative therapeutic approach. Cellarity was founded by Flagship Pioneering, a team of entrepreneurial scientists that conceives, creates, resources, and grows first-in-category life sciences companies. Since 2000, Flagship has created over 75 groundbreaking companies that are pioneering novel and proprietary biological, industrial, and engineering approaches to solve major needs in human health and sustainability. These companies include Moderna Therapeutics (NASDAQ:MRNA), Seres Therapeutics (NASDAQ:MCRB), Syros Pharmaceuticals (NASDAQ:SYRS), Rubius Therapeutics (NASDAQ:RUBY), Evelo Biosciences (NASDAQ:EVLO), Kaleido Biosciences (NASDQ:KLDO), and Indigo Agriculture. Cellarity is a highly dynamic, entrepreneurial, and innovation-driven organization seeking to hire an exceptional research associate to join our newly established Platform Development team. Position Summary: Cellarity is seeking an innovative and highly motivated Senior Research Associate with expertise in cell and molecular biology and assay development to play a vital role in the company's efforts to validate novel target discovery approaches. The candidate will be responsible for independently designing and developing cell-based models to demonstrate our ability to therapeutically manipulate healthy and diseased tissues. The position requires strong experimental skills particularly in the development of quantitative assays and in the culture of primary cells, stem cells and cancer cell lines. The successful candidate will develop fit-for-purpose assays designed to critique and improve our platform methods but will also collaborate with scientists for assay development in other exploratory and therapeutic groups. They will be comfortable working in, and contributing to, a very dynamic and cross-functional team environment. The ideal candidate must be independent, goal oriented, have excellent communication and organizational skills and be comfortable developing assays using a range of measurements and techniques. Education and Training: BS/MS degree in cell biology or related discipline 3 - 8 years Biotech/Pharma experience in cell and molecular biology and drug discovery Preferred Qualifications: Proven hands-on experience with cell-based and molecular biology assays with a variety of readout technologies, such as, but not limited to: cell culture, flow cytometry, high content imaging, qPCR, ELISA, and Western analysis Extensive experience with mammalian cell culture techniques, including the generation of stable and inducible cell lines Experience with characterization and/or differentiation of cells from human and animal tissues including hematopoietic cells, intestinal, epithelial, muscle and/or endothelial tissue is required Experience independently generating precise, reliable data and skillfully analyzing the data Excellent communication and presentation skills, capable of conveying technical information in a clear and thorough manner Motivated and excited about biological discovery and assay development Flagship Pioneering is committed to equal employment opportunity regardless of race, color, ancestry, religion, sex, national origin, sexual orientation, age, citizenship, marital status, disability, gender identity or Veteran status. Recruitment & Staffing Agencies : Flagship Pioneering and its affiliated Flagship Lab companies (collectively, "FSP") do not accept unsolicited resumes from any source other than candidates. The submission of unsolicited resumes by recruitment or staffing agencies to FSP or its employees is strictly prohibited unless contacted directly by Flagship Pioneering's internal Talent Acquisition team. Any resume submitted by an agency in the absence of a signed agreement will automatically become the property of FSP, and FSP will not owe any referral or other fees with respect thereto.
01/25/2021
Full time
Company Summary: Cellarity, Inc. is a privately held, well-funded, early-stage biotechnology company that is pioneering a novel transformative therapeutic approach. Cellarity was founded by Flagship Pioneering, a team of entrepreneurial scientists that conceives, creates, resources, and grows first-in-category life sciences companies. Since 2000, Flagship has created over 75 groundbreaking companies that are pioneering novel and proprietary biological, industrial, and engineering approaches to solve major needs in human health and sustainability. These companies include Moderna Therapeutics (NASDAQ:MRNA), Seres Therapeutics (NASDAQ:MCRB), Syros Pharmaceuticals (NASDAQ:SYRS), Rubius Therapeutics (NASDAQ:RUBY), Evelo Biosciences (NASDAQ:EVLO), Kaleido Biosciences (NASDQ:KLDO), and Indigo Agriculture. Cellarity is a highly dynamic, entrepreneurial, and innovation-driven organization seeking to hire an exceptional research associate to join our newly established Platform Development team. Position Summary: Cellarity is seeking an innovative and highly motivated Senior Research Associate with expertise in cell and molecular biology and assay development to play a vital role in the company's efforts to validate novel target discovery approaches. The candidate will be responsible for independently designing and developing cell-based models to demonstrate our ability to therapeutically manipulate healthy and diseased tissues. The position requires strong experimental skills particularly in the development of quantitative assays and in the culture of primary cells, stem cells and cancer cell lines. The successful candidate will develop fit-for-purpose assays designed to critique and improve our platform methods but will also collaborate with scientists for assay development in other exploratory and therapeutic groups. They will be comfortable working in, and contributing to, a very dynamic and cross-functional team environment. The ideal candidate must be independent, goal oriented, have excellent communication and organizational skills and be comfortable developing assays using a range of measurements and techniques. Education and Training: BS/MS degree in cell biology or related discipline 3 - 8 years Biotech/Pharma experience in cell and molecular biology and drug discovery Preferred Qualifications: Proven hands-on experience with cell-based and molecular biology assays with a variety of readout technologies, such as, but not limited to: cell culture, flow cytometry, high content imaging, qPCR, ELISA, and Western analysis Extensive experience with mammalian cell culture techniques, including the generation of stable and inducible cell lines Experience with characterization and/or differentiation of cells from human and animal tissues including hematopoietic cells, intestinal, epithelial, muscle and/or endothelial tissue is required Experience independently generating precise, reliable data and skillfully analyzing the data Excellent communication and presentation skills, capable of conveying technical information in a clear and thorough manner Motivated and excited about biological discovery and assay development Flagship Pioneering is committed to equal employment opportunity regardless of race, color, ancestry, religion, sex, national origin, sexual orientation, age, citizenship, marital status, disability, gender identity or Veteran status. Recruitment & Staffing Agencies : Flagship Pioneering and its affiliated Flagship Lab companies (collectively, "FSP") do not accept unsolicited resumes from any source other than candidates. The submission of unsolicited resumes by recruitment or staffing agencies to FSP or its employees is strictly prohibited unless contacted directly by Flagship Pioneering's internal Talent Acquisition team. Any resume submitted by an agency in the absence of a signed agreement will automatically become the property of FSP, and FSP will not owe any referral or other fees with respect thereto.
Sana Biotechnology
South San Francisco, California
JOB PURPOSE Sana Biotechnology has an exciting opportunity for a Senior Research Associate within the Cell Therapy Process Development group. The Senior Research Associate will focus on the development of a novel chimeric antigen receptor T (CAR T) cell manufacturing process. Under the supervision of the process development lead, the candidate will perform experiments to support the development of multiple unit operations including cell selection and expansion, gene editing, and cryopreservation. The Senior Research Associate is expected to closely collaborate with multiple teams including Analytical Development. DUTIES AND RESPONSIBILITIES Perform small- and large-scale cell therapy experiments using primary cells across multiple unit operations including cell selection, expansion, gene editing, cell harvest and cryopreservation Perform in-process analysis of samples including cell counting and metabolite analysis Document all experimental work in an electronic laboratory notebook Analyze data and present conclusions to a multidisciplinary group Draft technical documents including reports, operating procedures and master batch records Execute various parts of established processes by adhering to established procedures QUALIFICATIONS Basic Qualifications BSc. in Biology, Biochemistry or related field plus 4 yrs or MSc. plus 1-2 yrs or equivalent combination of education and work experience >2 yrs of experience with mammalian cell culture Excellent aseptic cell culture technique and good scientific method Detail-oriented with good problem-solving, technical writing and verbal communication skills Ability to effectively work in multidisciplinary teams, meet deadlines, and prioritize multiple projects Aptitude for working in a self-driven, performance/results-oriented, fast-paced matrix environment Ability to engage in crucial conversations - providing and receiving feedback The candidate should be a team player, highly organized with excellent communication skills, demonstrate curiosity and scientific rigor and excellent problem-solving skills Preferred Qualifications Prior experience working on a clinical or commercial cell therapy manufacturing process Knowledge of immunology with focus on T cell biology Experience with cell therapy unit operations including: Cell selection T cell culture Viral and non-viral gene editing methods Cell washing and harvesting Cryopreservation Experience with analytical methods including: Cell counts and viability using automated cell counters Media metabolite analysis Flow cytometry Cell-based assays, including Incucyte system Demonstrate technical curiosity and a continuous drive to learn new skills Experience following and drafting controlled documents Knowledge of R, Python, JMP, Spotfire, Benchling and FlowJo software WORKING CONDITIONS This position requires handling of human blood products Some weekend work required Work in a laboratory environment approximately 75% of the time PHYSICAL REQUIREMENTS Able to lift up to 25 lb.
01/25/2021
Full time
JOB PURPOSE Sana Biotechnology has an exciting opportunity for a Senior Research Associate within the Cell Therapy Process Development group. The Senior Research Associate will focus on the development of a novel chimeric antigen receptor T (CAR T) cell manufacturing process. Under the supervision of the process development lead, the candidate will perform experiments to support the development of multiple unit operations including cell selection and expansion, gene editing, and cryopreservation. The Senior Research Associate is expected to closely collaborate with multiple teams including Analytical Development. DUTIES AND RESPONSIBILITIES Perform small- and large-scale cell therapy experiments using primary cells across multiple unit operations including cell selection, expansion, gene editing, cell harvest and cryopreservation Perform in-process analysis of samples including cell counting and metabolite analysis Document all experimental work in an electronic laboratory notebook Analyze data and present conclusions to a multidisciplinary group Draft technical documents including reports, operating procedures and master batch records Execute various parts of established processes by adhering to established procedures QUALIFICATIONS Basic Qualifications BSc. in Biology, Biochemistry or related field plus 4 yrs or MSc. plus 1-2 yrs or equivalent combination of education and work experience >2 yrs of experience with mammalian cell culture Excellent aseptic cell culture technique and good scientific method Detail-oriented with good problem-solving, technical writing and verbal communication skills Ability to effectively work in multidisciplinary teams, meet deadlines, and prioritize multiple projects Aptitude for working in a self-driven, performance/results-oriented, fast-paced matrix environment Ability to engage in crucial conversations - providing and receiving feedback The candidate should be a team player, highly organized with excellent communication skills, demonstrate curiosity and scientific rigor and excellent problem-solving skills Preferred Qualifications Prior experience working on a clinical or commercial cell therapy manufacturing process Knowledge of immunology with focus on T cell biology Experience with cell therapy unit operations including: Cell selection T cell culture Viral and non-viral gene editing methods Cell washing and harvesting Cryopreservation Experience with analytical methods including: Cell counts and viability using automated cell counters Media metabolite analysis Flow cytometry Cell-based assays, including Incucyte system Demonstrate technical curiosity and a continuous drive to learn new skills Experience following and drafting controlled documents Knowledge of R, Python, JMP, Spotfire, Benchling and FlowJo software WORKING CONDITIONS This position requires handling of human blood products Some weekend work required Work in a laboratory environment approximately 75% of the time PHYSICAL REQUIREMENTS Able to lift up to 25 lb.
Summary 4DMT is in need of a talented and motivated Senior Research Associate to join our Bioinformatics team in supporting our viral drug therapy research efforts as we contribute to the future of gene therapeutics. The position will combine modern molecular and cell biology methods with scalable production processes to inform and improve discovery and experimental design in conjunction with 4DMT's proprietary discovery platform. This position will involve new protocol development and execution in the areas of AAV plasmid construction, high-throughput AAV production, purification and characterization, and cell-based optimization of payload biology. This highly-collaborative position will require close interaction on multiple projects within the Bioinformatics group to explore the wetlab aspects of computationally-derived hypotheses, and across the Discovery & Engineering department, including capsid discovery, payload engineering, and viral manufacturing. This position will also collaborate on cross-functional projects with additional groups within R&D and across the company, including HCDM, CMC, and Translational Medicine. As part of this cross-functional work, employee will be expected to attend and actively engage in working group and project team meetings. Responsibilities Research - 100% of Time Demonstrating innovative design, development, and execution of research projects through expertise and scientific teamwork Contributing to the design, implementation, and interpretation of the results of experimental studies Leveraging a strong understanding of gene therapy principles to design, assess and interpret experimental data, then using the results to inform the selection of the optimal product strategy Maintaining current awareness of scientific literature Actively applying new concepts and technologies Leading and supporting research initiatives such as new technology development and platform improvement projects Writing study reports and manuscripts and presenting results at scientific meetings Molecular biology in support of: AAV Library construction Monitoring of selections Novel screening strategies Next-generation sequencing (NGS) sample prep and MiSeq operation Nextera resequencing of plasmids and AAV genomes Refine and execute proprietary NGS-based assays for AAV sequence integrity and purity Maintaining compliance with company Environmental Health and Safety policies, procedures, and practices Contributing to building a culture that embraces continuous learning, improvement, and innovation and encourages team members to expand their technical skill base and deepen their gene therapy expertise. Qualifications BS/MS with a molecular and/or cell biology focus Experience: 5+ years industry experience Strong molecular biology experience, both hands-on as well as the ability to design and analyze sequence data using common software such as Geneious Extensive cell culture experience, with work in a plate-based format a plus Familiarity with NGS sample prep a plus Skills: (non-technical and technical skills) Successful demonstrated ability to conduct research with strong attention to detail and rigorous analysis and interpretation of data Ability to handle large-scale and parallel experiments in an organized and efficient manner Ability to multi-task and support more than one project simultaneously Highly organized and motivated; strong analytical, organizational, and problem-solving skills Strong work ethic, flexibility, and cooperative can-do attitude Proven interest and ability to build a culture that embraces continuous learning, improvement, and innovation Ability to work cooperatively in a team-based environment; supportive of multiple viewpoints and approaches Strong communication skills (both written and oral) with demonstrated ability to present ideas, information and data effectively via one on one discussions, team meetings, and external partnership interactions Physical Requirements of the Role: During COVID-19 will this person need to be in a lab? Yes How many days will be required? Full time Adhere to 4DMT Covid protocols and policy 4DMT provides equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, gender, sexual orientation, national origin, age, disability, genetic information, marital status, status as a covered veteran and any other category protected under applicable federal, state, provincial and local laws. Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities
01/24/2021
Full time
Summary 4DMT is in need of a talented and motivated Senior Research Associate to join our Bioinformatics team in supporting our viral drug therapy research efforts as we contribute to the future of gene therapeutics. The position will combine modern molecular and cell biology methods with scalable production processes to inform and improve discovery and experimental design in conjunction with 4DMT's proprietary discovery platform. This position will involve new protocol development and execution in the areas of AAV plasmid construction, high-throughput AAV production, purification and characterization, and cell-based optimization of payload biology. This highly-collaborative position will require close interaction on multiple projects within the Bioinformatics group to explore the wetlab aspects of computationally-derived hypotheses, and across the Discovery & Engineering department, including capsid discovery, payload engineering, and viral manufacturing. This position will also collaborate on cross-functional projects with additional groups within R&D and across the company, including HCDM, CMC, and Translational Medicine. As part of this cross-functional work, employee will be expected to attend and actively engage in working group and project team meetings. Responsibilities Research - 100% of Time Demonstrating innovative design, development, and execution of research projects through expertise and scientific teamwork Contributing to the design, implementation, and interpretation of the results of experimental studies Leveraging a strong understanding of gene therapy principles to design, assess and interpret experimental data, then using the results to inform the selection of the optimal product strategy Maintaining current awareness of scientific literature Actively applying new concepts and technologies Leading and supporting research initiatives such as new technology development and platform improvement projects Writing study reports and manuscripts and presenting results at scientific meetings Molecular biology in support of: AAV Library construction Monitoring of selections Novel screening strategies Next-generation sequencing (NGS) sample prep and MiSeq operation Nextera resequencing of plasmids and AAV genomes Refine and execute proprietary NGS-based assays for AAV sequence integrity and purity Maintaining compliance with company Environmental Health and Safety policies, procedures, and practices Contributing to building a culture that embraces continuous learning, improvement, and innovation and encourages team members to expand their technical skill base and deepen their gene therapy expertise. Qualifications BS/MS with a molecular and/or cell biology focus Experience: 5+ years industry experience Strong molecular biology experience, both hands-on as well as the ability to design and analyze sequence data using common software such as Geneious Extensive cell culture experience, with work in a plate-based format a plus Familiarity with NGS sample prep a plus Skills: (non-technical and technical skills) Successful demonstrated ability to conduct research with strong attention to detail and rigorous analysis and interpretation of data Ability to handle large-scale and parallel experiments in an organized and efficient manner Ability to multi-task and support more than one project simultaneously Highly organized and motivated; strong analytical, organizational, and problem-solving skills Strong work ethic, flexibility, and cooperative can-do attitude Proven interest and ability to build a culture that embraces continuous learning, improvement, and innovation Ability to work cooperatively in a team-based environment; supportive of multiple viewpoints and approaches Strong communication skills (both written and oral) with demonstrated ability to present ideas, information and data effectively via one on one discussions, team meetings, and external partnership interactions Physical Requirements of the Role: During COVID-19 will this person need to be in a lab? Yes How many days will be required? Full time Adhere to 4DMT Covid protocols and policy 4DMT provides equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, gender, sexual orientation, national origin, age, disability, genetic information, marital status, status as a covered veteran and any other category protected under applicable federal, state, provincial and local laws. Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities
JOB PURPOSE Sana Biotechnology is looking to expand our team with hard-working individuals who believe in the potential of cells as engineered medicines, have the vision to do creative things, and the determination to execute upon that vision. As a member of the In Vitro Pharmacology and Biomarker Discovery group, you will work within a highly dynamic and cross-functional environment on projects of diverse scope and across multiple therapeutic areas. To accelerate the development of transformative medicines, we seek a Senior Research Associate to build essential platform capabilities as well as develop and implement functional assays to support biomarker discovery, IND enabling studies, platform and research programs. The Senior Research Associate will perform in vitro characterizations of lead gene and cell therapy candidates in complex pharmacological and clinically relevant models. He/She will have experience culturing a diverse array of primary cell types and experience establishing and phenotypically characterizing in vitro models of disease. The successful candidate will have demonstrated technical proficiency, exhibit scientific rigor and independent thought as well as the ability to thrive in a team-oriented environment. DUTIES AND RESPONSIBILITIES Uses professional concepts in accordance with company objectives to solve problems in creative and effective ways. Culture and maintain multiple primary cell types including but not limited to hepatocytes, T-cells, PBMC's, splenocytes, cardiomyocytes, as well as endothelial and stromal cell types. Establish and validate in vitro functional assays using a variety of platforms (high-content imaging, incucyte, flow cytometry, ELISA, MSD, Luminex, HTRF, qPCR) Responsible for thoughtful planning, coordination and execution of experiments. Makes detailed observations, interpret results and document experimental methods in a well-maintained laboratory notebook. Proven ability to work independently. Presents results at team meetings. Facilitates discussion with other team members as necessary to ensure the completion of the team's projects. Comply with standard laboratory practices and company policies QUALIFICATIONS Basic Qualifications Master's degree or Bachelor's degree with 3-4+ years of laboratory experience in an academic or industry setting and proven proficiency performing independent research. Extensive hands-on experience culturing mammalian cell lines (primary and immortalized) with demonstrated competence in sterile cell culture technique and standard laboratory procedures. Experience developing and characterizing in vitro models of disease. Experience in the development of cell-based assays using a variety of platforms (Incucyte, High-content imaging, TR-FRET, ELISA, MSD, Flow Cytometry, Luminex, qPCR). Ability to proactively engage groups with diverse functions within a highly matrixed organization. Excellent oral and written communication skills, attention to detail and data quality, and experience working on cross-functional teams. Technical proficiency, scientific creativity, independent thought and ability to actively communicate with scientists throughout the organization. Preferred Qualifications Industry experience Experience with primary cell culture is highly preferred. Research experience in liver biology and/or immunology. Experience characterizing metabolic pathways and disease modeling
01/23/2021
Full time
JOB PURPOSE Sana Biotechnology is looking to expand our team with hard-working individuals who believe in the potential of cells as engineered medicines, have the vision to do creative things, and the determination to execute upon that vision. As a member of the In Vitro Pharmacology and Biomarker Discovery group, you will work within a highly dynamic and cross-functional environment on projects of diverse scope and across multiple therapeutic areas. To accelerate the development of transformative medicines, we seek a Senior Research Associate to build essential platform capabilities as well as develop and implement functional assays to support biomarker discovery, IND enabling studies, platform and research programs. The Senior Research Associate will perform in vitro characterizations of lead gene and cell therapy candidates in complex pharmacological and clinically relevant models. He/She will have experience culturing a diverse array of primary cell types and experience establishing and phenotypically characterizing in vitro models of disease. The successful candidate will have demonstrated technical proficiency, exhibit scientific rigor and independent thought as well as the ability to thrive in a team-oriented environment. DUTIES AND RESPONSIBILITIES Uses professional concepts in accordance with company objectives to solve problems in creative and effective ways. Culture and maintain multiple primary cell types including but not limited to hepatocytes, T-cells, PBMC's, splenocytes, cardiomyocytes, as well as endothelial and stromal cell types. Establish and validate in vitro functional assays using a variety of platforms (high-content imaging, incucyte, flow cytometry, ELISA, MSD, Luminex, HTRF, qPCR) Responsible for thoughtful planning, coordination and execution of experiments. Makes detailed observations, interpret results and document experimental methods in a well-maintained laboratory notebook. Proven ability to work independently. Presents results at team meetings. Facilitates discussion with other team members as necessary to ensure the completion of the team's projects. Comply with standard laboratory practices and company policies QUALIFICATIONS Basic Qualifications Master's degree or Bachelor's degree with 3-4+ years of laboratory experience in an academic or industry setting and proven proficiency performing independent research. Extensive hands-on experience culturing mammalian cell lines (primary and immortalized) with demonstrated competence in sterile cell culture technique and standard laboratory procedures. Experience developing and characterizing in vitro models of disease. Experience in the development of cell-based assays using a variety of platforms (Incucyte, High-content imaging, TR-FRET, ELISA, MSD, Flow Cytometry, Luminex, qPCR). Ability to proactively engage groups with diverse functions within a highly matrixed organization. Excellent oral and written communication skills, attention to detail and data quality, and experience working on cross-functional teams. Technical proficiency, scientific creativity, independent thought and ability to actively communicate with scientists throughout the organization. Preferred Qualifications Industry experience Experience with primary cell culture is highly preferred. Research experience in liver biology and/or immunology. Experience characterizing metabolic pathways and disease modeling